MicroRNA-101-3p Suppresses mTOR and Causes Mitochondrial Fragmentation and Cell Degeneration in COPD

Figure 5

The effect of miR-101-3p on mTOR signaling pathway. (a) Concentration-dependent modification of phosphorylation (p-)p70S6K by miR-101-3p mimics in ASMC of healthy nonsmokers (control, n = 3). (b) Concentration-dependent modification of phosphorylation (p-)p70S6K by miR-101-3p inhibitors in COPD-ASMC (n = 3). (c) Comparison of the p-/t- p70S6K ratio in five Western blots of ASMC from healthy nonsmokers (control) to COPD by image analysis (Figures 5(a) and 5(b)). Bars represent mean ± S.E.M. values were calculated by comparison to untreated cells. (d) Comparison of the t-p70S6K/GAPDH ratio in five Western blots in ASMC from healthy nonsmokers (control) to COPD by image (Figures 5(a) and 5(b)). Bars represent mean ± S.E.M.

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