MicroRNA-383-5p Regulates Oxidative Stress in Mice with Acute Myocardial Infarction through the AMPK Signaling Pathway via PFKM

Figure 4

miR-383-5p reduces the effects of oxidative stress through the AMPK signaling pathway. The myocardial tissues used for following detections were, respectively, treated with sham, AMI+PBS, AMI+miR-383-5p agomir, and AMI+miR-383-5p agomir NC. (a, b) Protein expression of AMPK and p-AMPK, in the myocardial tissue of mice by western blot analysis. (c) MDA kit was used to detect the expression level of MDA in the 4 groups. (d) Flow cytometry was used to detect ROS levels in the 4 groups of myocardial tissues. vs. the sham group; ^# vs. the AMI+PBS; AMPK and p-AMPK protein expressions were regarded as measurement data, presented by and analyzed by one-way ANOVA. There were 10 mice in each group, and the experiment was repeated 3 times; miR-383-5p: microRNA-383-5p; AMPK: AMP-activated protein kinase; p-AMPK: phosphorylated AMP-activated protein kinase; MDA: malondialdehyde; ROS: reactive oxygen species.

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