Research Article
TOPK Affects Autophagy of Skin Squamous Cell Carcinoma by Regulating NF-KB Pathway through HDAC1
Figure 8
The effect of TOPK silencing/overexpression on autophagy. The autophagosomes were observed by TEM. Compared with the control and no-load group S, the autophagosomes decreased in SCL-1 cells of siTOPK group while increased in A431 cells of TOPK OE group. Compared with TOPK OE group, the number of autophagy bodies decreased in PDTC group and 3-MA group; Western Blot revealed that the expressions of Beclin-1 and LC3II/I decreased significantly in SCL-1 cells of siTOPK group () and increased significantly in A431 cells of TOPK OE group (); compared with TOPK OE group, the protein expression levels of Beclin-1 and LC3II/I decreased significantly in PDTC group and 3-MA group (). See Figure 8(c) and 8(d). vs. control, vs. TOPK OE NC or siTOPK-NC, and vs. TOPK OE.
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