The Effect of Cordycepin on Steroidogenesis and Apoptosis in MA-10
Mouse Leydig Tumor Cells
Figure 7
A1, A2A, A2B, and A3 AR were involved in cordycepin-induced cell death in MA-10 mouse Leydig tumor cells. MA-10 cells were treated with A1, A2A, A2B, and A3 AR agonists in the absence or presence of cordycepin for 24 hr. (a) shows that MA-10 cells were incubated in the absence or presence of cordycepin (100 μM and 1 mM) for 24 hr. (b) shows that MA-10 cells were incubated in AR agonists (A1 100 μM, A2A 25 μM, A2B 100 μM, and A3 50 μM) for 24 hr. (c) shows that MA-10 cells were incubated in cordycepin (100 μM or 1 mM) plus AR agonists (A1 10 μM, A2A 10 μM, A2B 10 μM, and A3 50 μM) for 24 hr. Cell morphology was observed and recorded under light microscopy (Olympus, CK 40). Note the same magnification (200x) among Figures. Arrowhead (▲) indicates rounded-up cells and/or membrane-blebbed cells. MA-10 cells (5,000 cells/well) were treated with different dosages (1~100 μM) of A1-, A2A-, A2B-, and A3-AR agonist in the absence (d) or presence of cordycepin (100 μM and 1 mM) for 24 hr (e). MTT assay was used to assess cell viability, which was proportional to absorbance at 590 nm. Each data point in the Figure represents the mean the mean ± SEM of four independent experiments with triplicates in each treatment. Asterisk *() above the bars indicates significant difference compared with control. #() above the bars indicates significant difference compared with cordycepin alone treatment group (C: cordycepin).
