Aqueous Extract of Paeonia suffruticosa Inhibits Migration and Metastasis of Renal Cell Carcinoma Cells via Suppressing VEGFR-3 Pathway
Figure 2
Anticancer effects of PS-A on RCC cells. (a) 7 × 102 of 786O cells were treated with various concentrations of PS-A for 1, 2, and 3 days. The rate of cell proliferation was measured by MTT assay. The data are represented as the means ± SD (). (b) 2.5 × 104 of 786O cells were seeded in transwell chambers and incubated in serum-free medium containing 0, 0.3, and 0.6 mg/mL of PS-A for 12 h. The migrated cells on the bottom of transwell chambers were fixed and then stained. The migratory activity was determined by counting migrated cells in three microscopic fields. The data are represented as the means ± SD (). *; ** compared with untreated cells. (c) Fully confluent 786O cells were scrapped by P200 pipette tips and then treated with 0, 0.3, and 0.6 mg/mL of PS-A. Images were taken at 0 and 6 h after scrapping under 100x magnification. (d) 2.5 × 104 of 786O cells were seeded in matrigel-coated chambers and then incubated in serum-free medium containing 0, 0.3, and 0.6 mg/mL of PS-A for 18 h. The invaded cells were fixed and then stained. The invasive activity was determined by counting invaded cells in three microscopic fields. The data are represented as the means ± SD (). *; ** compared with untreated cells.
