Evidence-Based Complementary and Alternative Medicine / 2012 / Article / Fig 4

Research Article

Aqueous Extract of Paeonia suffruticosa Inhibits Migration and Metastasis of Renal Cell Carcinoma Cells via Suppressing VEGFR-3 Pathway

Figure 4

Suppression of VEGFR-3/FAK/Rac-1 signaling by PS-A. (a) After serum starvation for 24 h, 786O cells were treated with or without 0.3 mg/mL of PS-A for 24 h, and the lysates were analyzed by Western blot analysis to detect the expression levels of VEGFR-3 and the phosphorylated status of FAK. Actin was used as the loading control. The intensities of bands were quantitated and normalized to actin levels. (b) After serum starvation for 24 h, 786O cells were treated with or without 0.3 mg/mL of PS-A for 12 h, and the lysates were incubated with GST-PBD beads to examine the levels of GTP-bound Rac-1 (upper). The levels of total Rac-1 and actin in total lysates were analyzed by Western blotting (lower).
409823.fig.004a
(a)
409823.fig.004b
(b)

We are committed to sharing findings related to COVID-19 as quickly and safely as possible. Any author submitting a COVID-19 paper should notify us at help@hindawi.com to ensure their research is fast-tracked and made available on a preprint server as soon as possible. We will be providing unlimited waivers of publication charges for accepted articles related to COVID-19. Sign up here as a reviewer to help fast-track new submissions.