Aqueous Extract of Paeonia suffruticosa Inhibits Migration and Metastasis of Renal Cell Carcinoma Cells via Suppressing VEGFR-3 Pathway
Figure 4
Suppression of VEGFR-3/FAK/Rac-1 signaling by PS-A. (a) After serum starvation for 24 h, 786O cells were treated with or without 0.3 mg/mL of PS-A for 24 h, and the lysates were analyzed by Western blot analysis to detect the expression levels of VEGFR-3 and the phosphorylated status of FAK. Actin was used as the loading control. The intensities of bands were quantitated and normalized to actin levels. (b) After serum starvation for 24 h, 786O cells were treated with or without 0.3 mg/mL of PS-A for 12 h, and the lysates were incubated with GST-PBD beads to examine the levels of GTP-bound Rac-1 (upper). The levels of total Rac-1 and actin in total lysates were analyzed by Western blotting (lower).
