Herbal Extracts Combination (WNK) Prevents Decline in Spatial Learning and Memory in APP/PS1 Mice through Improvement of Hippocampal Aβ Plaque Formation, Histopathology, and Ultrastructure
Figure 2
Light micrographs of HE-stained CA1 hippocampal neurons (×400). (a) Nontransgenic group; (b) transgenic group; (c) WNK group; (d): EGB761 group. Neurons in nontransgenic mouce brain exhibited well-defined shapes and nucleus (Figure 2(a)). Damaged neurons in transgenic one are darkly stained and exhibited shrunken and triangulated neuronal body (black arrows). Cells are arranged in disorder with a slightly changed cell polarity. Cytoplasmic swelling, neuron loss, and microglia hyperplasia (white arrows) can also be seen (Figure 2(b)). In WNK- or EGB761-treated brains, neurons display smoothly stained regularly spaced pear-shaped cell bodies, containing large, round, or oval nuclei, similar to the nontransgenic ones, while cytoplasmic swelling and vacuolation are observed (arrow heads) (Figures 2(c) and 2(d)).
