The Molecular Basis of Wound Healing Processes Induced by Lithospermi Radix: A Proteomics and Biochemical Analysis
Figure 2
2D PAGE analysis of the protein differentially expressed in human fibroblast after treatment with the LR extracts or shikonin. Fibroblasts were treated with 5 μg/mL LR extract, 20 μg/mL LR extract, or 100 nM shikonin for 24 h, and then the whole cell lysates were collected and analyzed by 2D PAGE using a pH 4–7 IEF strips and 12.5% SDS-PAGE. The resulting gel was then visualized by silver staining. The treatments of the cells for the 2D PAGE are indicated below each gel and are (a) 0.5% of DMSO, (b) 5 μg/mL LR extract (LR5), (c) 20 μg/mL LR extract (LR20), and (d) 100 nM of shikonin (S100). It should be noted that there were 22 differentially expressed protein spots with ratio >1.5 or <0.8 fold compared to the DMSO control that were identified from LR5-, LR20-, and S100-treated cells. The corresponding protein spot identities are shown in Table 1.
