Evidence-Based Complementary and Alternative Medicine

Evidence-Based Complementary and Alternative Medicine / 2012 / Article
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Medical Ethnobiology and Ethnopharmacology in Latin America

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Research Article | Open Access

Volume 2012 |Article ID 846583 | https://doi.org/10.1155/2012/846583

Edilson Alves dos Santos, Cenira M. de Carvalho, Ana L. S. Costa, Adilva S. Conceição, Flávia de B. Prado Moura, Antônio Euzébio Goulart Santana, "Bioactivity Evaluation of Plant Extracts Used in Indigenous Medicine against the Snail, Biomphalaria glabrata, and the Larvae of Aedes aegypti", Evidence-Based Complementary and Alternative Medicine, vol. 2012, Article ID 846583, 9 pages, 2012. https://doi.org/10.1155/2012/846583

Bioactivity Evaluation of Plant Extracts Used in Indigenous Medicine against the Snail, Biomphalaria glabrata, and the Larvae of Aedes aegypti

Academic Editor: Ulysses Paulino De Albuquerque
Received12 Jul 2011
Revised02 Sep 2011
Accepted15 Sep 2011
Published11 Dec 2011


This investigation examined the molluscicidal and larvicidal activity of eight plants that are used in the traditional medicine of the Pankararé indigenous people in the Raso da Catarina region, Bahia state, Brazil. The tested plants were chosen based on the results of previous studies. Only those plants that were used either as insect repellents or to treat intestinal parasitic infections were included in the study. Crude extracts (CEs) of these plants were tested for their larvicidal activity (against Aedes aegypti larvae in the fourth instar) and molluscicidal activity (against the snail Biomphalaria glabrata). The plant species Scoparia dulcis and Helicteres velutina exhibited the best larvicidal activities (LC50 83.426 mg/L and LC50 138.896 mg/L, resp.), and Poincianella pyramidalis, Chenopodium ambrosoides, and Mimosa tenuiflora presented the best molluscicidal activities (LC50 0.94 mg/L, LC50 13.51 mg/L, and LC50 20.22 mg/L, resp.). As we used crude extracts as the tested materials, further study is warranted to isolate and purify the most active compounds.

1. Introduction

The Brazilian northeast is the poorest region of Brazil and has the worst Human Development Indices [1]. Most of this population is subjected to neglected tropical diseases that predominantly affect the poorest and most vulnerable groups, contributing to the perpetuation of poverty, inequality, and social exclusion [2].

Schistosomiasis and dengue fever cause major public health concerns in Brazil and other tropical developing countries. Schistosomiasis is caused by the parasite, Schistosoma mansoni, which uses the Biomphalaria glabrata snail as an essential intermediate host in its life cycle. Dengue fever is caused by an arbovirus of the Flaviviridae family and is transmitted by the mosquito, Aedes aegypti.

The number of cases of dengue has grown in Brazil, with epidemics in the most densely populated urban areas. However, natural products with different biocidal activities can help to fight parasite vectors at the adult or larval stages and can act as alternatives to synthetic products due to their rapid biodegradation and lower cost [3].

Molluscicides have been used as a general strategy to eliminate the snail that transmits schistosomiasis [4]. According to the World Health Organization (WHO), the use of drug therapy in conjunction with the use of molluscicides is the use the most valuable method to control schistosomiasis in areas with intermediary hosts. The synthetic substance, niclosamide (Bayluscide), has been used as the standard molluscicide since the 1960s, as it is efficient in controlling snails; however, the high cost of niclosamide and the fact that it decomposes rapidly in the presence of sunlight have limited the use of this drug [5].

Popular knowledge has been an important source of information for scientific research in several areas of study. Ethnopharmacological and ethnobotanical investigations have been used as the main strategy for selecting medicinal plants, thereby shortening the time for the discovery of new drugs, whereas ethnodirected research consists of selecting species based on information from population groups [6].

Evidence for the efficacy and safety and the immediate availability of plant-derived products for the control or eradication of such diseases would be of great value because part of the population living in the affected areas use plants and animals as one of the few options for disease treatment [79].

Studies have found evidence that standard methods control the dengue-related mosquito larvae with low efficacy, a situation that demonstrates the need for other means to fight the proliferation of dengue [10] given the fact that results in epidemiology are context-dependent [11]. Similarly, despite the fact that a national schistosomiasis control program was implemented in 1975, the disease still occurs in 19 states and is endemic to eight states.

Ethnobiological studies have been carried out on the indigenous Pankararé people since 1993 [12]. In 2006 [13], the use of 64 plants was reported, 20 of which were used for medicinal purposes. Indeed, there is evidence that the Pankararé—in the Estação Ecologica Raso da Catarina (a conservation area), Bahia state—have a profound knowledge regarding the benefits of plants.

This study examines the molluscicidal and larvicidal effects of eight plants used by the Pankararé indigenous people for medicinal purposes. The aim of the study is to look for evidence of alternative methods to fight vectors of schistosomiasis and dengue, taking into account local potentialities.

2. Materials and Methods

2.1. Study Area and Population

The indigenous lands of the Pankararé are located in one of the driest of the Brazilian regions, with an average annual rainfall of between 450 and 600 mm [14] and an average annual temperature of 25°C; the climate is arid and semiarid. The natural vegetation is tropical dry forest of the type hyperxerophylous steppic savanna.

The Pankararé have a long history of interaction with their regional neighbors and are a peasant social group that sees itself as a distinct ethnic group among the regional populations (from the social organization standpoint, this is termed Indigenous Peasantry). In Brazilian indigenous communities, the central political figure is the Cacique [15]. The Pankararé comprise a very poor group that has a long history of territorial disputes. They practice subsistence agriculture, farm livestock on a small scale, and engage in other activities, such as hunting, the collection of honey and wild fruits, and handicrafts [16].

2.2. Ethnobotanical Survey and Plant Collection

Ethnobiologic studies have been carried out on the Pankararé indigenous people since 1993. However, the present study was based mainly on recent information [17], specifically, on studies that were conducted in 2008 and 2009. The ethnobotanical research was carried out after the community members were fully informed of its purpose and they had granted their permission to record information. Authorization was also obtained from the community Cacique and the National Foundation of Support to the Indians (FUNAI). The data were collected using some of the methods presented and discussed in a previous report [18], including the use of informal conversations and semistructured interviews with 35 residents of the indigenous community (9 men and 26 women, aged 23 to 64 years). The interviewees were identified using the snowball technique based on the information initially provided by the traditional specialists, that is, people that are knowledgeable about medicinal plants.

For the taxonomic identification of the species, we collected reproductive plants that were donated to and identified in the Herbarium of the University of Bahia State UNEB (HUNEB-Coleção, Paulo Afonso).

2.3. Obtaining the Extracts

Eight plants were tested in bioassays to evaluate their larvicidal and molluscicidal activities. Plants that are used as insect repellents or anthelmintics were included in the study. To obtain the extracts, botanical material was gathered between March 2008 and February 2009. Material of the collected species (roots, stems, stem bark, and leaves) was dried at room temperature and then ground to powder. From this powder, we obtained the crude extract (CE) of each material by extracting three consecutive times with 90% ethanol for 72 h in a stainless steel extractor at 27 ± 1°C followed by the remotion of the solvent in a rotary evaporator at 60°C. The concentrated and weighed crude extracts were bioassayed to evaluate their larvicidal and molluscicidal activities at the Institute of Chemistry and Biotechnology (IQB) at the Federal University of Alagoas, UFAL.

2.4. Evaluation of the Molluscicidal Activity

Individual snails of the species Biomphalaria glabrata (of diameter 13–18 mm and obtained from the Institute René Rachou in Belo Horizonte city) were housed in aquaria equipped with continuously circulating dechlorinated water, at the temperature of 28°C, in the bioassay laboratory of the IQB of UFAL, based on the protocol of Santos and Sant’ana [19]. The ethanolic extracts of the stems, stem bark, roots, and leaves of the plants were dissolved in an aqueous solution of Dimethyl Sulfoxide (DMSO 0.1%) to obtain various concentrations (0.10–100 μg/mL). The assay consisted of immersing five snails in 125 mL of the dissolved test extract for 24 hours. After this period, the snails were washed, placed in dechlorinated water, and fed. The snails remained under observation for a further 24 hours, and the dead snails were recorded and removed. The extracts that exhibited molluscicidal activity at concentrations lower than 100 μg/mL in the duplicate bioassays were considered active and subjected to further tests. A total of 10 snails was used in these tests (maintaining the ratio of 25 mL of test solution per snail), and each concentration was tested in triplicate. In parallel, we carried out control tests using an aqueous solution of 0.1% DMSO with the molluscicide, niclosamide, at 3 μg/mL. We used the program Probit, version 1.5 [55] to calculate the LC50.

The World Health Organization (WHO) has indicated that the crude extracts of plants presenting LC50 values of <40 ppm (0.04% and 0.4 μg/mL) have potential as larvicidal and molluscicidal compounds [56].

2.5. Evaluation of the Larvicidal Activity

The larvicidal activity was tested on the larvae of the mosquito Aedes aegypti in the bioassay laboratory of the IQB/UFAL, based on methodology described by the WHO [57]. The crude extracts were diluted in aqueous solutions of 1% DMSO to 500 μg/mL. Then, 10 larvae of A. aegypti in the fourth instar stage were prepared for immersion in the crude extract (20 mL). The larvae were counted 24 hours and 48 hours from the beginning of the experiment, and the tests were performed in quadruplicate. For the control, we used an aqueous solution of 1% DMSO containing 3 μg/mL of the synthetic larvicide, Temephos. The activity of the tested extracts was established based on the average percentage of mortality of the larvae after 48 hours (>75% [promising result], 50–75% [partially promising], 25–50% [weakly promising], and <25% [inactive]).

2.6. Data Analysis

The results are reported as the average of three repetitions ( ) ± the standard deviation of the mean. The values found were subjected to ANOVA and Tukey’s test ( ). All of the analyses were performed using the Microcal Origin, version 8.0, and GraphPad Prism, version 5.0, programs.

3. Results and Discussion

The eight plant species examined belong to six families and eight genera, and a biological activity has previously been described in the literature for these species (Table 1). These species were cited by the Pankararé as insect repellents (5 species) and/or anthelmintics (4 species). The analyses of the extracts and the root, stem, and leaf fractions demonstrated that seven (87.5%) of the eight tested species presented activity. Five species (62.5%) were active against the mollusk, Biomphalaria glabrata, and two species (25%) were active against larvae of the Aedes aegypti mosquito.

Popular nameFamily/SpeciesPart usedBiological activityIsolated substancesReferences

Pinhão brancoBeuphorbiaceae/
Jatropha mollissima (Pohl) Baill.
Leaves, stem bark, and rootsAntioxidant * [20]

Jurema pretaBfabaceae/
Mimosa tenuiflora (Willd.) Poir.
Leaves, stem bark, and roots * Kukulkanins A and B[21]

Croton moritibensis Baill.
Leaves and aerial parts * 12-hydroxy-13-methyl-1,8,11,13-podocarpatetraen-3-one, 2-ethoxycarbonyltetrahydroharman and hydroxy-2-methyltetrahydroharman[22]

Scoparia dulcis L.
Aerial partsAntidiabetic, antioxidant, cytotoxic, antiulcer, antileishmania, and anti-hyperlipidemicScoparic acid D[2332]
Helicteres velutina K. Schum.
Aerial partsCytotoxic against various cancer lineages**Cucurbitacins D and J**[33]
Chenopodium ambrosioides L.
Aerial partsInhibitor of the mitochondrial electron transport chain (toxicity), antiparasitic, antihyperperistalsis, anti-inflammatory, analgesic, antischistosomiasis, antituberculosis, larvicidal, antitumor, and antifungalCaryophyllene oxide[3444].
Poincianella pyramidalis (Tul.) L.P. Queiroz
Leaves, stem bark and rootsAntimicrobial, antioxidant and antifungal4,4′-dihydroxy-2′-methoxy-chalcone[4548].
Hyptis pectinata L.
Aerial partsAntimicrobial, antinociceptive, anti-inflammatory, hepatoprotective, antibacterial, and antifungalCalamusenone-antimicrobial[4954].

* No studies were found in the literature; **activity has been described for another species of the same genus; Alarvicidal activity; Bmolluscicidal activity.

Two of the seven species with activity (Chenopodium ambrosioides L. and Hyptis pectinata Poit.) are cultivated in home gardens. The remaining five species (Helicteres velutina K. Schum., Jatropha mollissima (Pohl) Baill., Poincianella pyramidalis (Tul.) L.P. Queiroz, Mimosa tenuiflora (Willd.) Poir., and Scoparia dulcis L.) are native or ruderal species.

The species with the strongest activity against the larvae of A. aegypti was S. dulcis, whose ethanolic leaf extract presented an LC50 83.426 mg/L. The next strongest activity was presented by Helicteres velutina K. Schum., with an LC50 of 138.89 mg/mL and 171.68 mg/L, respectively, for the extracts obtained from the stems (215 g) and roots (208 g) (Table 2).

Tested speciesPopular namePart usedLC10LC50LC90

Scoparia dulcisVassourinhaLeaf43.82083.426158.829
HelicteresvelutinaPitóRoot73. 029171.683403.607

Previous studies report that the extracts from the roots and stems of S. dulcis present a high activity against the bacterium Klebsiella pneumoniae and gram-negative bacteria [45]. Such results may be associated with the presence of steroids, saponins, polyphenols, and glutinol [58]. Flavonoids were also described as occurring in the aerial parts of S. dulcis [59], which suggests the presence of antioxidant activity. S. dulcis is a good source of betulinic acid (Figure 1), a compound with proven anticarcinogenic, antimelanoma, antiviral, and cytotoxic properties. Many biological activities that were found in S. dulcis have been attributed to various compounds, including scopadulcic acids A and B, scopadiol, scopadulciol, and scopadulin [60] (Figure 1).

No data were found in the literature that could be used in a comparative analysis of Helicteres velutina, a species whose roots and stems exhibited activity in the present study; such previously reported data were found only for Helicteres angustifolia L. [33]. The other species tested did not present activity against the larvae of A. aegypti using the applied method.

P. pyramidalis (Tul.) L.P. (LC50 0.94 mg/L), C. ambrosoides (LC50 13.51 mg/L), M. tenuiflora (LC50 20.22 mg/L), H. pectinata (LC50 25.55 mg/L), and J. molissima (LC50 33.55 mg/L) demonstrated the strongest molluscicidal activities (Table 3). The extracts of C. moritibensis (the stems, roots, leaves, stem bark, and root bark) did not exhibit any significant activity, as based on the recommendations of the WHO [56]. These recommendations propose that only the aqueous and alcoholic extracts of plants that promote the death of 90% of the animals when tested at concentrations equal to or lower than 20 mg/L (over a 24-hour exposure) should be tested in the field and deserve attention in studies on the purification and isolation of the most active compounds.

Crude extractCommon nameUsed partLC10LC50LC90

Jatropha mollissimaPinhão-brancoStem20.0033.5556.26
Hyptis pectinataSambacaetáLeaf8.4825.3475.66
Poincianella pyramidalisCatingueiraLeaf0.040.9420.03
Chenopodium ambrosoidesMastruzLeaf1.9913.5191.57
Mimosa tenuifloraJurema pretaStem6.5920.2262.05

The ethanolic extract of P. pyramidalis has been used successfully against resistant strains of Escherichia coli (strain ATCC25922) and Staphylococcus aureus (ATCC25923) [48]. A phytochemical study of this species revealed the presence of phenolic compounds, such as glucosyl-phenylpropanoid acid, 4-Obd-glucopyranosyloxi-Z-7-hydroxycinnamic acid and 4-O-b-glucopyranosyloxi-Z-8-hydroxycinnamic acid, and flavonoids agathisflavone, apigenin, and kaempferol [53] (Figure 2).

The excellent efficacy of this species is important, and the crude extract deserves further studies with regard to the purification and isolation of the most active compounds. Importantly, P. pyramidalis occurs frequently and this species grows densely in semiarid dry forest in northeastern Brazil, locally known as caatinga [61], presenting the strong potential for large-scale exploitation without incurring a major risk to its population and the possibility of generating income for local communities and creating major public health benefits.

Mastruz (C. ambrosoides), which was the second most efficacious species, provides an essential oil containing the active ingredient monoterpene ascaridol [62]. The essential oil of this species has been used as an anthelmintic in humans but, due to its high toxicity, it has been replaced with safer drugs [63].

In a review on the phytochemistry of jurema-preta, Souza et al. [64] report the presence of several metabolites, such as alkaloids, chalcones, steroids, terpenoids, and phenoxychromones. Choi et al. [65] has suggested that many biological plant activities are due to the presence of total phenols, such as flavonoids and tannins.

Sambacaitá (H. pectinata), which is also bioactive, has proven anti-inflammatory, hepatoprotective, antibacterial, antifungal, and antinociceptive action [4954].

The bark extract from J. mollissima was more toxic to the A. aegypti larvae than the leaf or root extracts. These results are similar to those found by Heal et al. [66].

The diterpenoids, jatrophone, and jatropholones A and B (Figure 3), are classified as cytotoxic substances, and the isolation of tumor growth inhibitors from this plant has been cited by Goulart et al. [67] and Kupchan et al. [68, 69]. These substances have been the subject of several scientific studies and have been reported to inhibit the effects of insulin [70].

Although previous researchers have studied the bioactive substances of seven of the eight species examined here, no reference was found concerning its molluscicidal or larvicidal potential.

Although there is a need for further investigation to isolate and identify the constituents of the crude plant extracts studied, the results of this study provide evidence that some of the plants tested contain natural insecticides and have the potential for larvicidal activity and may provide substitutes for synthetic products in the future. Developments in this direction may lead to important positive impacts on public health programs, particularly with regard to socially excluded populations.

4. Conclusion

During the molluscicidal tests, we verified that the leaf extracts of Poincianella pyramidalis and Chenopodium ambrosoides were very effective and had a high level of activity. However, other extracts were more promising for larvicidal activity; of particular note were the leaf extracts from Scoparia dulcis and the stem and root extracts of Helicteres velutina. Based on these results, the plants of the studied region have proven to be a rich source of substances with larvicidal activity (based on Aedes aegypti larvae in the fourth instar) and molluscicidal activity (based on the snail Biomphalaria glabrata). Further attention is warranted for the purification and isolation of the most active compounds from these extracts, and field tests are needed. From the information presented here, the need for an interdisciplinary research program in the northeastern semiarid region is evident, especially in the village of the Pankararé people. Such a program would be aimed at developing the sustainable use of natural resources and promoting community development.


The authors wish to express their gratitude to the indigenous community of the Pankararé (Cacique Afonso and Patrícia) and to Professor Charles Estevão from the Laboratory of Biochemistry and Chemistry of Natural Products (LBPN) of UFS, FUNAI, FUNASA, UNEB, CNPq, and CAPES.


  1. Programa das Nações Unidas para o Desenvolvimento, “A verdadeira riqueza das nações: vias para o desenvolvimento humano,” 2010, http://www.pnud.org.br/rdh. View at: Google Scholar
  2. P. Hotez, “A new voice for the poor,” PLoS Neglected Tropical Diseases, vol. 1, no. 1, article e77, 2007. View at: Publisher Site | Google Scholar
  3. A. Marston and K. Hostettmann, “Plant molluscicides,” Phytochemistry, vol. 24, no. 4, pp. 639–652, 1985. View at: Google Scholar
  4. A. R. Bilia, A. Braca, J. Mendez, and I. Morelli, “Molluscicidal and piscicidal activities of Venezuelan chrysobalanaceae plants,” Life Sciences, vol. 66, no. 4, pp. 53–59, 2000. View at: Google Scholar
  5. V. S. Delgado, D. P. Suarez, I. M. Cesari, and R. N. Incani, “Experimental chemotherapy of Schistosoma mansoni with praziquantel and oxamniquine: differential effect of single or combined formulations of drugs on various strains and on both sexes of the parasite,” Parasitology Research, vol. 78, no. 8, pp. 648–654, 1992. View at: Google Scholar
  6. U. P. Albuquerque and N. Hanazaki, “As pesquisas etnodirigidas na descoberta de novos fármacos de interesse médico e farmacêutico: fragilidades e pespectivas,” Revista Brasileira de Farmacognosia, vol. 16, 2006. View at: Publisher Site | Google Scholar
  7. A. F. dos Santos, M. R. F. De Lima, M. C. De Omena et al., “A study of the larvicidal and molluscicidal activities of some medicinal plants from northeast Brazil,” Journal of Ethnopharmacology, vol. 97, no. 2, pp. 199–206, 2005. View at: Publisher Site | Google Scholar
  8. L. T. G. Ruiz, E. G. Magalhães, A. F. Magalhães et al., “Avaliação da atividade tóxica em Artemia salina e Biomphalaria glabrata de extratos de quatro espécies do gênero Eleocharis (Cyperaceae),” Revista Brasileira de Farmacognosia, vol. 15, no. 2, 2005. View at: Publisher Site | Google Scholar
  9. R. R. N. Alves, J. A. A. Barbosa, S. L. D. X. Santos, W. M. S. Souto, and R. R. D. Barboza, “Animal-based remedies as complementary medicines in the semi-arid region of northeastern Brazil,” Evidence-Based Complementary and Alternative Medicine, vol. 2011, Article ID 179876, 15 pages, 2011. View at: Publisher Site | Google Scholar
  10. F.M Garelli, M. O. Espinosa, D. Weinberg, M. A. Trinelli, and R. E. Gürtler, “Water use practices limit the effectiveness of a temephos-based Aedes aegypti larval control program in northern Argentina,” PLoS Neglected Tropical Diseases, vol. 3, no. 5, p. 991, 2011. View at: Google Scholar
  11. R. Bhopal, “Seven mistakes and potential solutions in epidemiology, including a call for a World Council of Epidemiology and Causality,” Emerging Themes in Epidemiology, vol. 6, article 6, 2009. View at: Publisher Site | Google Scholar
  12. F. P. S. F. Bandeira, Etnobiologia Pankararé. Salvador. Monografia De Bacharelado em Ciências Biológicas, Instituto de Biologia da UFBA, 1993.
  13. M. A. D. S. Colaço, Etnobotânica dos índios Pankararé, no Raso da Catarina—Bahia: Uso e Importância Cultural de Plantas da Caatinga, M.S. thesis, Feira de Santana, Bahia, Brazil, 2006.
  14. Ministério das Minas e Energia Secretaria Geral, “Projeto RADAMBRASIL: folha SC.24/25 Aracaju/Recife; geologia, geomorfologia, pedologia, vegetação e usopotencial da terra,” Rio de Janeiro, Brasil: 856p., il., 7 mapas, (Levantamento de Recursos Naturais, 30), 1983. View at: Google Scholar
  15. M. S. Maia, Os Pankararé do Brejo do Burgo Capesinato e Etnicidade. Monografia do Bacharelado e Antropologia, Universidade Federal da Bahia, 1992.
  16. F. P. F. Bandeira, Diagnóstico Etnoambiental da Terra indígena Pankararé, UEFS, 2003.
  17. E. A. Santos, Análise fitoquimica e ensaios biológicos de plantas da caatinga utilizada pelos índios pankarare da estação ecológica do raso da catarina, Ph.D. thesis, Programa de Pós Graduação em Quimica e Biotecnologia, Universidade Federal de Alagoas, 2011.
  18. U. P. Albuquerque and R. F. P. Lucena, Métodos Etécnicas na Pesquisa Etnobotânica, Livro Rápido/NUPPEA, Recife, Brasil, 2004.
  19. A. F. dos Santos and A. E. G. Sant'ana, “Molluscicidal activity of the diterpenoids jatrophone and jatropholones A and B isolated from Jatropha elliptica (Pohl) Muell. Arg,” Phytotherapy Research, vol. 13, no. 8, pp. 660–664, 1999. View at: Publisher Site | Google Scholar
  20. J. G. De Melo, T. A. De Sousa Araújo, V. T. N. De Almeida Castro et al., “Antiproliferative activity, antioxidant capacity and tannin content in plants of semi-arid northeastern Brazil,” Molecules, vol. 15, no. 12, pp. 8534–8542, 2010. View at: Publisher Site | Google Scholar
  21. X. A. Dominguez, S. G. Garcia, H. J. Williams, C. Ortiz, A. I. Scott, and J. H. Reibenspies, “Kukulkanins A and B, new chalcones from Mimosa tenuefolia,” Journal of Natural Products, vol. 52, no. 4, pp. 864–867, 1989. View at: Google Scholar
  22. V. T. De Araújo-Júnior, M. S. Da Silva, E. V. Leitao Da-Cunha et al., “Alkaloids and diterpenes from Croton moritibensis,” Pharmaceutical Biology, vol. 42, no. 1, pp. 62–67, 2004. View at: Publisher Site | Google Scholar
  23. M. A. Adaikpoh, N. E. J. Orhue, and I. Igbe, “The protective role of Scoparia dulcis on tissue antioxidant defense system of rats exposed to cadmium,” African Journal of Biotechnology, vol. 6, no. 10, pp. 1192–1196, 2007. View at: Google Scholar
  24. M. Ahsan, S. K. N. Islam, A. I. Gray, and W. H. Stimson, “Cytotoxic diterpenes from Scoparia dulcis,” Journal of Natural Products, vol. 66, no. 7, pp. 958–961, 2003. View at: Publisher Site | Google Scholar
  25. M. Babincová, K. Schronerová, and P. Sourivong, “Antiulcer activity of water extract of Scoparia dulcis,” Fitoterapia, vol. 79, no. 7-8, pp. 587–588, 2008. View at: Publisher Site | Google Scholar
  26. J. E. Beh, J. Latip, M. P. Abdullah, A. Ismail, and M. Hamid, “Scoparia dulcis (SDF7) endowed with glucose uptake properties on L6 myotubes compared insulin,” Journal of Ethnopharmacology, vol. 129, no. 1, pp. 23–33, 2010. View at: Publisher Site | Google Scholar
  27. M. S. Gachet, J. S. Lecaro, M. Kaiser et al., “Assessment of anti-protozoal activity of plants traditionally used in Ecuador in the treatment of leishmaniasis,” Journal of Ethnopharmacology, vol. 128, no. 1, pp. 184–197, 2010. View at: Publisher Site | Google Scholar
  28. M. Latha, L. Pari, S. Sitasawad, and R. Bhonde, “Scoparia dulcis, a traditional antidiabetic plant, protects against streptozotocin induced oxidative stress and apoptosis in vitro and in vivo,” Journal of Biochemical and Molecular Toxicology, vol. 18, no. 5, pp. 261–272, 2004. View at: Publisher Site | Google Scholar
  29. M. Latha, L. Pari, K. M. Ramkumar et al., “Antidiabetic effects of scoparic acid D isolated from scoparia dulcis in rats with streptozotocin-induced diabetes,” Natural Product Research, vol. 23, no. 16, pp. 1528–1540, 2009. View at: Publisher Site | Google Scholar
  30. S. Mesía-Vela, M. Bielavsky, L. M. B. Torres et al., “In vivo inhibition of gastric acid secretion by the aqueous extract of Scoparia dulcis L. in rodents,” Journal of Ethnopharmacology, vol. 111, no. 2, pp. 403–408, 2007. View at: Publisher Site | Google Scholar
  31. L. Pari and M. Latha, “Protective role of Scoparia dulcis plant extract on brain antioxidant status and lipidperoxidation in STZ diabetic male Wistar rats,” BMC Complementary and Alternative Medicine, vol. 4, article 16, 2004. View at: Publisher Site | Google Scholar
  32. L. Pari and M. Latha, “Antihyperlipidemic effect of Scoparia dulcis (Sweet Broomweed) in streptozotocin diabetic rats,” Journal of Medicinal Food, vol. 9, no. 1, pp. 102–107, 2006. View at: Publisher Site | Google Scholar
  33. Z. T. Chen, S. W. Lee, and C. M. Chen, “Cucurbitacin B 2-sulfate and cucurbitacin glucosides from the root bark of Helicteres angustifolia,” Chemical and Pharmaceutical Bulletin, vol. 54, no. 11, pp. 1605–1607, 2006. View at: Publisher Site | Google Scholar
  34. J. L. Bezerra, Avaliação da atividade leishmanicida de espécies vegetais, M.S. thesis, Universidade Federal do Maranhão, 2006.
  35. F. Calzada, R. Arista, and H. Pérez, “Effect of plants used in Mexico to treat gastrointestinal disorders on charcoal-gum acacia-induced hyperperistalsis in rats,” Journal of Ethnopharmacology, vol. 128, no. 1, pp. 49–51, 2010. View at: Publisher Site | Google Scholar
  36. G. F. Ibironke and K. I. Ajiboye, “Studies on the anti-inflammatory and analgesic properties of Chenopodium ambrosioides leaf extract in rats,” International Journal of Pharmacology, vol. 3, no. 1, pp. 111–115, 2007. View at: Google Scholar
  37. E. G. Kamel, M. A. El-Emam, S. S. M. Mahmoud, F. M. Fouda, and F. E. Bayaumy, “Attenuation of Schistosoma mansoni cercarial infectivity to albino mice by methanol extract of some plant species,” Pesticide Biochemistry and Physiology, vol. 98, no. 3, pp. 342–348, 2010. View at: Publisher Site | Google Scholar
  38. F. Kiuchi, Y. Itano, N. Uchiyama et al., “Monoterpene hydroperoxides with trypanocidal activity from Chenopodium ambrosioides,” Journal of Natural Products, vol. 65, no. 4, pp. 509–512, 2002. View at: Publisher Site | Google Scholar
  39. N. Lall and J. J. M. Meyer, “In vitro inhibition of drug-resistant and drug-sensitive strains of Mycobacterium tuberculosis by ethnobotanically selected South African plants,” Journal of Ethnopharmacology, vol. 66, no. 3, pp. 347–354, 1999. View at: Publisher Site | Google Scholar
  40. F. Massebo, M. Tadesse, T. Bekele, M. Balkew, and T. Gebre-Michael, “Evaluation on larvicidal effects of essential oils of some local plants against Anopheles arabiensis Patton and Aedes aegypti Linnaeus (Diptera, Culicidae) in Ethiopia,” African Journal of Biotechnology, vol. 8, no. 17, pp. 4183–4188, 2009. View at: Google Scholar
  41. L. Monzote, W. Stamberg, K. Staniek, and L. Gille, “Toxic effects of carvacrol, caryophyllene oxide, and ascaridole from essential oil of Chenopodium ambrosioides on mitochondria,” Toxicology and Applied Pharmacology, vol. 240, no. 3, pp. 337–347, 2009. View at: Publisher Site | Google Scholar
  42. F. R. F. Nascimento, G. V. B. Cruz, P. V. S. Pereira et al., “Ascitic and solid Ehrlich tumor inhibition by Chenopodium ambrosioides L. treatment,” Life Sciences, vol. 78, no. 22, pp. 2650–2653, 2006. View at: Publisher Site | Google Scholar
  43. F. J. Patrício, G. C. Costa, P. V. S. Pereira et al., “Efficacy of the intralesional treatment with Chenopodium ambrosioides in the murine infection by Leishmania amazonensis,” Journal of Ethnopharmacology, vol. 115, no. 2, pp. 313–319, 2008. View at: Publisher Site | Google Scholar
  44. C. S. Prasad, R. Shukla, A. Kumar, and N. K. Dubey, “In vitro and in vivo antifungal activity of essential oils of Cymbopogon martini and Chenopodium ambrosioides and their synergism against dermatophytes,” Mycoses, vol. 53, no. 2, pp. 123–129, 2009. View at: Publisher Site | Google Scholar
  45. W. S. Alviano, D. S. Alviano, C. G. Diniz et al., “In vitro antioxidant potential of medicinal plant extracts and their activities against oral bacteria based on Brazilian folk medicine,” Archives of Oral Biology, vol. 53, no. 6, pp. 545–552, 2008. View at: Publisher Site | Google Scholar
  46. M. V. Bahia, J. B. dos Santos, J. P. David, and J. M. David, “Biflavonoids and other phenolics from Caesalpinia pyramidalis (Fabaceae),” Journal of the Brazilian Chemical Society, vol. 16, no. 6 B, pp. 1402–1405, 2005. View at: Google Scholar
  47. M. C. S. Cruz, P. O. Santos, A. M. Barbosa et al., “Antifungal activity of Brazilian medicinal plants involved in popular treatment of mycoses,” Journal of Ethnopharmacology, vol. 111, no. 2, pp. 409–412, 2007. View at: Publisher Site | Google Scholar
  48. M. R. F. De Lima, J. de Souza Luna, A. F. dos Santos et al., “Anti-bacterial activity of some Brazilian medicinal plants,” Journal of Ethnopharmacology, vol. 105, no. 1-2, pp. 137–147, 2006. View at: Publisher Site | Google Scholar
  49. M. F. Arrigoni-Blank, A. R. Antoniolli, L. C. Caetano, D. A. Campos, A. F. Blank, and P. B. Alves, “Antinociceptive activity of the volatile oils of Hyptis pectinata L. Poit. (Lamiaceae) genotypes,” Phytomedicine, vol. 15, no. 5, pp. 334–339, 2008. View at: Publisher Site | Google Scholar
  50. M. D. Bispo, R. H. V. Mourão, E. M. Franzotti et al., “Antinociceptive and antiedematogenic effects of the aqueous extract of Hyptis pectinata leaves in experimental animals,” Journal of Ethnopharmacology, vol. 76, no. 1, pp. 81–86, 2001. View at: Publisher Site | Google Scholar
  51. A. C. C. D. Lisboa, I. C. M. Mello, R. S. Nunes et al., “Antinociceptive effect of Hyptis pectinata leaves extracts,” Fitoterapia, vol. 77, no. 6, pp. 439–442, 2006. View at: Publisher Site | Google Scholar
  52. G. B. Melo, R. L. Silva, V. A. Melo et al., “Enhancement of liver regeneration by the association of Hyptis pectinata with laser therapy,” Digestive Diseases and Sciences, vol. 50, no. 5, pp. 949–954, 2005. View at: Publisher Site | Google Scholar
  53. P. F. C. Nascimento, W. S. Alviano, A. L. C. Nascimento et al., “Hyptis pectinata essential oil: chemical composition and anti-Streptococcus mutans activity,” Oral Diseases, vol. 14, no. 6, pp. 485–489, 2008. View at: Publisher Site | Google Scholar
  54. P. O. Santos, M. D. J. C. Costa, J. A. B. Alves et al., “Chemical composition and antimicrobial activity of the essential oil of Hyptis pectinata (L.) Poit,” Quimica Nova, vol. 31, no. 7, pp. 1648–1652, 2008. View at: Publisher Site | Google Scholar
  55. EPA, 2004, http://www.epa.gov/nerleerd/stat2.htm#probit.
  56. World Health Organization (WHO), Tropical Disease Research, World Health Organization, Geneva, Switzerland, 1993.
  57. World Health Organization (WHO), “Instruction for determining the susceptibility or resistance of mosquito larvae to insecticides,” WHO/VBC/75.583, 1975. View at: Google Scholar
  58. S. M. De Farias Freire, J. A. Da Silva Emim, A. J. Lapa, C. Souccar, and L. M. Brandao Torres, “Analgesic and antiinflammatory properties of Scoparia dulcis L. extracts and glutinol in rodents,” Phytotherapy Research, vol. 7, no. 6, pp. 408–414, 1993. View at: Publisher Site | Google Scholar
  59. L. M. B. O. Torres, J. W. B. Sobrinho, K. R. S. Marques et al., “Compostos polares de scoparia dulcis (Scrophulariaceae),” in Proceedings of the 17th Reunião Anual da Sociedade Brasileira de Química, 7th Simpósio Nacional de Química Inorgânica, Caxambu, Brasil, 1994. View at: Google Scholar
  60. Raintree Brasil, 2010, http://www.raintree.com.br/produtos/ntense2.htm#Scene_1.
  61. E. V. S. B. Sampaio, “Fitossociologia,” in Pesquisa Botânica Nordestina: Progresso e Perspectivas, E. V. S. B. Sampaio, S. J. Mayo, and M. R. V. Barbosa, Eds., pp. 203–230, Sociedade Botânica do Brasil, Seção Regional de Pernambuco, 1996. View at: Google Scholar
  62. H. Lorenzi and F. J. A. Matos, Plantas Medicinais do Brasil: Nativas e Exóticas Cultivadas, Instituto Plantarum, Nova Odessa, São Paulo, Brazil, 2002.
  63. H. Paget, “The determination of ascaridole in chenopodium oil,” The Analyst, vol. 51, no. 601, pp. 170–176, 1926. View at: Publisher Site | Google Scholar
  64. R. S. O. de Souza, U. P. De Albuquerque, J. M. Monteiro, and E. L. C. De Amorim, “Jurema-Preta (Mimosa tenuiflora [Willd.] Poir.): a review of its traditional use, phytochemistry and pharmacology,” Brazilian Archives of Biology and Technology, vol. 51, no. 5, pp. 937–947, 2008. View at: Google Scholar
  65. Y. M. Choi, D. O. Noh, S. Y. Cho, H. J. Suh, K. M. Kim, and J. M. Kim, “Antioxidant and antimicrobial activities of propolis from several regions of Korea,” LWT—Food Science and Technology, vol. 39, no. 7, pp. 756–761, 2006. View at: Publisher Site | Google Scholar
  66. R. E. Heal, E. F. Rogers, R. T. Wallace, and O. Starnes, “A survey of plants for insecticidal activity,” Lloydia, vol. 13, no. 2, pp. 89–162, 1950. View at: Google Scholar
  67. M. O. F. Goulart, A. E. G. Sant’ana, R. A. Lima, S. H. Cavalcante, M. G. Carvalho, and F. R. Braz, “Fitoconstituintes químicos isolados de jatropha elliptica. Atribuição dos deslocamentos químicos dos Átomos de carbono e hidrogênio dos diterpenos jatrofolonas A e B,” Química Nova, vol. 16, 1993. View at: Google Scholar
  68. S. M. Kupchan, C. W. Sigel, M. J. Matz, C. J. Gilmore, and R. F. Bryan, “Structure and stereochemistry of jatrophone, a novel macrocyclic diterpenoid tumor inhibitor,” Journal of American Chemical Society, vol. 98, no. 8, pp. 2295–2300, 1976. View at: Google Scholar
  69. S. M. Kupchan, C. W. Sigel, M. J. Matz, J. A. S. Renauld, R. C. Haltiwanger, and R. F. Bryan, “Jatrophone, a novel macrocyclic diterpenoid tumor inhibitor from Jatropha gossypiifolia,” Journal of the American Chemical Society, vol. 92, no. 14, pp. 4476–4477, 1970. View at: Google Scholar
  70. F. V. Menezes, E. M. Carneiro, E. Delattre, and A. C. Boschero, “Inhibition of insulin release by Jatrophone,” Brazilian Journal of Medical and Biological Research, vol. 25, no. 3, pp. 305–307, 1992. View at: Google Scholar

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