Discovery of Endothelium and Mesenchymal Properties of Primo Vessels in the Mesentery
Figure 4
Double labeling of primo vessels in the mesentery with vWF/PDI and CD31/PDI. (a) Fluorescent microscopy of cryosections double-labeled with antibodies to vWF (green) and PDI (red). DAPI was used for labeling of nuclei (blue). In addition to the single-color panels, the pictures were merged. Primo vessel cells with rod-shaped nuclei and linear alignments were labeled positive for both vWF and PDI (yellow, white arrows). (b) CD31/PDI double labeling of primo vessels in the mesentery. Fluorescent microscopy of cryosections double-labeled with antibodies to CD31 (green) and PDI (red). DAPI was used for labeling of nuclei (blue). Accumulation of CD31/PDI positive cells (yellow, white arrows) correlated with areas of primo vessels with rod-shaped nuclei, linear alignments.
