Discovery of Endothelium and Mesenchymal Properties of Primo Vessels in the Mesentery
Figure 6
Immunofluorescent staining of primary primo vessel cells with endothelial markers and fibroblast markers. (a) Labeling of primo vessel cells with endothelial and fibroblast markers. Antibodies to endothelial markers (vWF, CD31, VE-cadherin) and fibroblast markers (PDI, MST1, procollagen I) (green) were used. Fluorescent microscopy revealed that both of endothelial markers and fibroblast markers (green) were expressed in primo vessel cells. (b) Controls for immunofluorescent staining of primo vessel cells. NIH3T3 cells and BHK-21 cells were positive to fibroblast markers (PDI, MTS1, procollagen I) (green) whereas negative to endothelial markers (vWF, CD31, VE-cadherin).
