Inhibitive Effects of Mulberry Leaf-Related Extracts on Cell Adhesion and Inflammatory Response in Human Aortic Endothelial Cells
Figure 4
Effects of MLREs on NF-κB activity. After HAECs were pretreated with the indicated samples and incubated with TNF-α, nuclear extracts were prepared, and the expression of NF-κB p65 was assessed by western blot analysis. A representative image of three similar results is shown (a). Actin served as the loading control for the nuclear compartment. Semiquantitative analysis of three independent experiments are also shown (b). # indicates a significant difference between the TNF-α treated or experimental treatment groups and the control, . * indicates a significant difference between the TNF-α and experimental treatment groups, BT, MT, and M at 25 and 50 μg/mL.