Real-time PCR analysis of expression of several virulence factor genes. MRSA ATCC 33591 was cultured and treated with sub-MIC concentrations (32–125 μg/mL) of U. pumila extract, and real-time PCR analysis was then performed as described in the Section 2. mecA, sea, sarA, and agrA expressions were significantly inhibited at concentration higher than 64 μg/mL. Each value is expressed as a mean ± standard deviation. Significance was determined at when compared with the control.