Vitamin K2, a Naturally Occurring Menaquinone, Exerts Therapeutic Effects on Both Hormone-Dependent and Hormone-Independent Prostate Cancer Cells
Figure 7
VK2 inhibits the expression of inflammatory cytokine genes: ~ LNCaP, DU145, and 22RV1 cells were incubated with 50 μM, 75 μM, and 100 μM Vk2, respectively, for 48 h at 37°C in 5% Co2 environment. Untreated cells served as control. Following incubation, RNA was extracted and converted to cDNA. Expressions of HMGB1, RAGE, IL-6, IL-8, VEGF-A, and androgen receptor (AR) genes were then quantified by real time PCR as indicated. Results presented are relative expression levels of genes in the treated samples compared to expression level of the untreated samples. Results show that Vk2 modulates the expression of inflammation related genes by down regulating AR, HMGB1, RAGE, IL-8 and VEGF-A in LNCaP cells ((a)–(c)) compared to control LNCaP cells (). In case of 22RV1 ((d)–(f)) and DU-145 (Figures 7(g)–7(i)) prostate cancer cells, VK2 inhibited RAGE, HMGB1, IL-6, IL-8, and VEGF-A expression compared to control cells (). Results presented are representative of one of three similar experiments.
