Neurite Outgrowth in PC12 Cells Stimulated by Components from Dendranthema × grandiflorum cv. “Mottenohoka” Is Enhanced by Suppressing Phosphorylation of p38MAPK
Figure 10
Effects of SB203580 on neurofilament-M expression of cultured PC12 cells induced by luteolin (B), acacetin (C), NGF, or EGF. PC12 cells were cultured and treated under the same conditions as shown in the legend of Figure 8. Cells were subsequently cultured for another 2 days in the serum-free medium. The expression of neurofilament-M of each sample was evaluated by immunoblotting. The intensity of each band was measured densitometrically by Image J. Values are expressed as the ratio of neurofilament-M to β-actin, and the means ± SEM of the values of three separate experiments are shown. Significant differences from the values of the corresponding control group were determined by ANOVA, followed by Dunnett’s test. Control group is signified as . *: significance difference at ; **: significant difference at ; —: no significant difference. Arrows indicate slight neurite outgrowth.
