Inhibitory Effect of Chrysanthemum zawadskii Herbich var. latilobum Kitamura Extract on RANKL-Induced Osteoclast Differentiation
Figure 5
Effect of CZE in RANKL-stimulated costimulatory signals in osteoclasts. BMMs were cultured with M-CSF and RANKL in the presence or absence of CZE (25 μg/mL) for 1 day. (a)-(b) RANKL-induced Ca2+-oscillation was measured with the Ca2+ indicator, Fura-2AM. (c) BMMs were cultured with M-CSF and RANKL in the presence of CZE for 1 day. RANKL-induced Ca2+-oscillation by acute treatment of CZE was measured. (d)-(e) BMMs were cultured with M-CSF and RANKL in presence or absence of CZE (25 μg/mL) for the indicated time. Lysate (30 μg) was subjected to SDS-PAGE, and the activation of PLCγ2 (d) and CREB (e) was analyzed by immunoblotting. All data are representative of at least three independent experiments.
