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Evidence-Based Complementary and Alternative Medicine
Volume 2013 (2013), Article ID 579319, 13 pages
http://dx.doi.org/10.1155/2013/579319
Review Article

Cichorium intybus: Traditional Uses, Phytochemistry, Pharmacology, and Toxicology

1Medical Research Council, HIV Prevention Research Unit, Westville, Durban 4041, South Africa
2School of Pharmaceutical Sciences, Lovely Professional University, Jalandhar-Delhi G.T. Road (NH-1), Phagwara, Punjab 144411, India
3Department of Agriculture and Animal health, University of South Africa (UNISA), Florida Campus, Florida 1710, South Africa

Received 2 August 2013; Revised 22 October 2013; Accepted 22 October 2013

Academic Editor: Young-Rae Lee

Copyright © 2013 Renée A. Street et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The genus Cichorium (Asteraceae) is made up of six species with major geographical presence in Europe and Asia. Cichorium intybus, commonly known as chicory, is well known as a coffee substitute but is also widely used medicinally to treat various ailments ranging from wounds to diabetes. Although this plant has a rich history of use in folklore, many of its constituents have not been explored for their pharmacological potential. Toxicological data on C. intybus is currently limited. This review focuses on the economic and culturally important medicinal uses of C. intybus. Traditional uses, scientific validation, and phytochemical composition are discussed in detail.

1. Introduction

The genus Cichorium (Asteraceae) consists of six species with major distribution areas in Europe and Asia [1]. In several Asteraceae, inulin, a β-2,1 linked fructose polymer with a terminal glucose residue, functions as a reserve carbohydrate in stems, tubers, and taproots [2]. Cichorium intybus L., commonly known as chicory, is an erect fairly woody perennial herb, around 1 m in height with a fleshy taproot of up to 75 cm in length and large basal leaves [1, 3]. Historically, chicory was grown by the ancient Egyptians as a medicinal plant, coffee substitute, and vegetable crop and was occasionally used for animal forage. In the 1970s, it was discovered that the root of C. intybus contained up to 40% inulin, which has a negligible impact on blood sugar and thus is suitable for diabetics [4]. To date, C. intybus is grown for the production of inulin on an industrial scale [2]. The name of the plant is derived from Greek and Latin. Cichorium means field and intybus is partly derived from the Greek “to cut”, because of the leaves, and partly from the Latin tubus to indicate the hollow stem [5].

Chicory is a hardy plant and can endure extreme temperatures during both vegetative and reproductive growth stages [1]. When broken, all plant parts exudate a milky latex [3]. Cichorium intybus is cultivated for numerous applications and can be divided into four main varieties or cultigroups according to their use [6]: (1) “industrial” or “root” chicory, predominantly cultivated in northwestern Europe, India, South Africa, and Chile, produces the taproot as a coffee substitute or for inulin extraction; (2) “Brussels” or “witloof” chicory is commonly cultivated around Europe as industrial chicory for etiolated buds (chicons) by forcing; (3) “leaf” chicory is used as fresh or cooked vegetables; and (4) “forage” chicory, initially derived from wild chicory commonly found along roadsides and waste areas, has been used since the mid-1970s to intensify herbage obtainability in perennial pastures for livestock.

Cichorium intybus is a medicinally important plant in Eurasia and in parts of Africa. Despite its long tradition of use, the plant is not described in the European Pharmacopoeia or in any official Pharmacopoeia of a European Union member state [5]. However, due to its prevalent distribution, different parts of the plant have been used in traditional medicines globally [7]. Important phytochemicals are distributed throughout the plant, but the main contents are present in the root [1]. This review focuses on the economic and culturally important medicinal uses of C. intybus. Traditional uses, scientific validation, and phytochemical composition are discussed in detail.

2. Traditional Uses

Medicinal plants have been used for centuries and numerous cultures still rely on indigenous medicinal plants to meet their primary health care needs. It is likely that the insightful knowledge of plant-based remedies in traditional cultures advanced through trial and error and that the most important cures were carefully passed from one generation to another [8]. Historically, chicory was grown by the ancient Egyptians as a medicinal plant [9] and it has had a long history of therapeutic use both in areas where it is indigenous and in areas where it has been introduced. The various common or local names describing this plant may be ascribed to the widespread use by different folkloric groups.

Different preparations of this plant are employed to treat various symptoms and ailments (Table 1). The juice is said to be a folk remedy for cancer of the uterus and for tumors [4]. In South Africa, although it is considered a widespread weed, leaves, stems, and roots are made into a tea for jaundice and chicory syrup is used as a tonic and purifying medicine for infants [3]. In Turkey, an ointment is made from the leaves for wound healing [10]. Decoction refers to a preparation that is made by adding cold water to the plant material which is then boiled and allowed to simmer for 5–10 min after which it is strained [8]. Chicory decoctions are traditionally made from individual plant parts and/or from the plant as a whole (Table 1).

tab1
Table 1: Traditional medicinal uses of Cichorium intybus.

According to the European monograph, traditional use of chicory roots includes the relief of symptoms related to mild digestive disorders (such as feeling of abdominal fullness, flatulence, and slow digestion) and temporary loss of appetite [11]. Prior to the wars in Afghanistan, folkloric reports described the use of aqueous root extracts as a light-sensitive plant remedy for malaria. This indigenous knowledge has since been confirmed and the antimalarial compounds of C. intybus roots have been identified as the light-sensitive sesquiterpene lactones lactucin and lactucopicrin [12]. The flowers of the chicory plant (Cichorii flos) are used as a herbal treatment of everyday ailments such as a tonic and appetite stimulant and as a treatment of gallstones, gastroenteritis, sinus problems, cuts, and bruises [4]. In Italy, the whorls are made into a decoction and used as a depurative [13]. Chicory seeds are one of the main ingredients of Jigrine, a commercial product of India used for the treatment of various diseases of the liver [14]. Other plant parts are also used for liver disorders, namely, aerial parts in Bosnia and Herzegovina [15] and roots in Serbia and India [16, 17].

3. Chemical Constituents

Chicoric acid has been identified as the major compound in methanolic extracts of chicory (Table 2) [18]. Aliphatic compounds and their derivatives comprise the main fraction while terpenoids comprise minor constituents of the plant. The flowers of chicory contain saccharides, methoxycoumarin cichorine, flavonoids, essential oils [4], and anthocyanins contributing to the blue colour of the perianth [19]. Table 2 provides a summary of the compounds isolated and identified from chicory. Octane, n-nonadecane, pentadecanone, hexadecane, and a tentatively identified compound have been found as principal volatile components [4]. A list of volatile compounds is given in Table 3.

tab2
Table 2: Compounds isolated and identified from Cichorium intybus (chicory).
tab3
Table 3: The volatile constituents of Cichorium intybus (adapted from Judžentienė and Būdienė [4]).

4. Pharmacological Activities

Cichorium intybus presents a little investigated plant in terms of phytochemistry and pharmacology. Over 100 individual compounds have been isolated and identified from this plant (Table 2), the majority of which are from the roots. Most of the pharmacological studies on this plant document the testing of aqueous and/or alcoholic extracts only. Apart from the pharmacologically important activities, the use of C. intybus (hairy root cultures) has also been implicated in the phytoremediation of DDT [20].

4.1. Antimicrobial Activity

The antibacterial activity of the organic acid-rich extract of fresh red chicory (C. intybus var. sylvestre) was tested against periodontopathic bacteria including Streptococcus mutans, Actinomyces naeslundii, and Prevotella intermedia. The compounds identified from the active extract include oxalic acid, succinic acid, quinic acid, and shikimic acid. All of the organic acids were found to decrease biofilm formation and adhesion of bacteria to the cells, with different levels of efficacy. These compounds also induced biofilm disruption and detachment of dead cells for the cultured substratum [21]. In other reports on the antimicrobial activity of C. intybus, the crude aqueous and organic seed extracts were found to be active against four pathogenic microorganisms, namely, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans, and root extracts had pronounced effects on Bacillus subtilis, S. aureus, Salmonella typhi, Micrococcus luteus, and E. coli [22, 23]. The leaf extract of C. intybus also showed a moderate activity against multidrug resistant S. typhi [24]. Guaianolides-rich root extracts of C. intybus have shown antifungal properties against anthropophilic fungi Trichophyton tonsurans, T. rubrum, and T. violaceum [25]. A sesquiterpenoid phytoalexin cichoralexin isolated from chicory exhibited potent antifungal activity against Pseudomonas cichorii [26].

4.2. Anthelmintic Activity

Several studies have been conducted on grazing animals to determine the anthelmintic potential of secondary metabolites present in C. intybus. Grossly, it has been concluded that the animals grazing on chicory have a higher performance index and lower incidence of gastrointestinal nematode infestations. In the majority of the experiments, the condensed tannins and sesquiterpene lactones were responsible for anthelmintic activity [27]. Anthelmintic activity of chicory has also been noticed in the case of lambs wherein the total number of abomasal helminths was found to be lesser in the lambs grazing on this plant [28]. The condensed tannin and sesquiterpene-rich extracts of C. intybus were evaluated for their efficacy against the larvae of deer lungworm, Dictyocaulus viviparous and other gastrointestinal nematode larvae using a larval migration inhibition assay. A dose-dependent decrease in the larval motility was observed in both lungworm and gastrointestinal nematodes [29]. The sesquiterpene lactone-rich extracts of C. intybus were also found to inhibit egg hatching of Haemonchus contortus [30].

4.3. Antimalarial Activity

The infusion of fresh roots of C. intybus has a history of use as a remedy for malarial fevers in some parts of Afghanistan. The bitter compounds in the plant, namely, lactucin, lactucopicrin, and the guaianolide sesquiterpenes, isolated from aqueous root extracts of chicory were concluded to be the antimalarial components of the plant. Lactucin and lactucopicrin completely inhibited the HB3 clone of strain Honduras-1 of Plasmodium falciparum at concentrations of 10 and 50 μg/mL, respectively [12, 31].

4.4. Hepatoprotective Activity

The folkloric use of C. intybus as a hepatoprotectant has been well documented. It is one of the herbal components of Liv-52, a traditional Indian tonic used widely for hepatoprotection. In a randomized, double-blind clinical trial conducted on cirrhotic patients, Liv-52 medication reduced the serum levels of hepatic enzymes, namely, alanine aminotransferase and aspartate aminotransferase. It also reduced the Child-Pugh scores and ascites significantly [32]. Another polyherbal formulation, Jigrine, contains the leaves of C. intybus as one of its 14 constituents. Jigrine was evaluated for its hepatoprotective activity against galactosamine-induced hepatopathy in rats. The pretreatment of male Wistar-albino rats with jigrine significantly reduced the levels of aspartate transaminase, alanine transaminase, and urea and increased the levels of blood and tissue glutathione. Histopathological examination of the liver revealed that jigrine pretreatment prevented galactosamine toxicity and caused a marked decrease in inflamed cells [33].

The aqueous-methanolic extract of the seeds of C. intybus has been investigated for the hepatoprotective activity against acetaminophen and carbon tetrachloride-induced liver damage in mice. It was found to decrease both the death rate and the serum levels of alkaline phosphatase, glutamyl oxaloacetate transaminase, and glutamyl pyruvate transaminase [34]. In analogous studies, the antihepatotoxic activity of the alcoholic extract of the seeds and aqueous extracts of the roots and root callus of C. intybus was estimated. The oral administration of these extracts in albino rats led to a marked decrease in the levels of hepatic enzymes. Also, histopathological examination of the liver showed no fat accumulation or necrosis after the treatment [14, 35]. Similar studies have established the hepatoprotective effect of esculetin, a phenolic compound, and cichotyboside, a guaianolide sesquiterpene glycoside reported from C. intybus [36, 37].

The carbon tetrachloride and paracetamol-induced liver toxicities were also found to be counteracted by intraperitoneal administration of crude extracts and fractions of C. intybus. The methanol- and water-soluble fractions exhibited marked reductions in serum glutamyl pyruvate transaminase, serum glutamyl oxaloacetate transaminase, alkaline phosphatase, and total bilirubin levels. In the same study, toxicity was induced in rat hepatocytes by incubation with galactosamine and thioacetamide [38].

The phenolic acid-rich seed extract of C. intybus was evaluated for its efficacy against hepatic steatosis in vitro and in vivo. The in vitro model of hepatic steatosis was created by incubation of the HepG2 cells with oleic acid leading to intracellular accumulation of fat. The seed extract was effective in decreasing the deposited fat from the cells in case of administration after the initial fat deposition (i.e., nonsimultaneous administration with oleic acid). However, in case of simultaneous administration of seed extract and oleic acid, the extract could not protect the cells from steatosis except at very high doses. The extract also led to the increased release of glycerol (an indicator of triglyceride degradation) in steatotic cells. In case of nonsimultaneous administration, the extract was found to upregulate the expression of SREBP-1c and PPAR-α genes leading to restoration of normal levels of corresponding proteins. In the in vivo model of hepatic steatosis, namely, diabetic rats, treatment with seed extract resulted in significant decrease in fat accumulation and fibrosis [39].The hepatoprotective activity of C. intybus has been correlated to its ability to inhibit the free radical mediated damage. A fraction prepared from the ethanolic extract of the leaves was assessed for preventive action on the free radical mediated damage to the deoxyribose sugar of the DNA (obtained from calf thymus). A dose-dependent decrease in the DNA damage was observed in the present assay [40].

4.5. Antidiabetic Activity

Chicory has reported antidiabetic activity [17, 41]. Based on the traditional use of C. intybus in diabetes mellitus, the hypoglycemic and hypolipidemic properties of the ethanol extract of the whole plant were investigated. Diabetes was induced by intraperitoneal administration of streptozotocin in male Sprague-Dawley rats. The ethanol extract, at a dose of 125 mg/Kg body weight, significantly attenuated the serum glucose levels in the oral glucose tolerance test. A marked decrease in the serum triglycerides and cholesterol was also observed in the extract-treated rats. Hepatic glucose-6-phosphatase activity was found to be reduced in extract-treated diabetic rats as compared to untreated diabetic rats [17]. The antidiabetic effect of the aqueous seed extract of C. intybus has also been investigated. Early-stage and late-stage diabetes were differently induced in male Wistar albino rats by streptozotocin-niacinamide and streptozotocin alone, respectively. The treatment with chicory extract prevented weight loss in both early-stage and late-stage diabetic rats. Chicory-treated diabetic animals resisted excessive increase in fasting blood sugar (assessed by glucose tolerance test). Grossly, normalization of blood parameters, namely, alanine aminotransferase, triacylglycerol, total cholesterol, and glycosylated heamoglobin, was seen in these animals. In early-stage diabetic rats, chicory treatment led to the increase in insulin levels pointing toward the insulin-sensitizing action of chicory [42].

Feeding the diabetic Wistar rats with C. intybus leaf powder led to a decrease in blood glucose levels to near normal value. C. intybus administration also decreased the malondialdehyde (formed by thiobarbituric acid) levels and increased glutathione content. Anticholinesterase activity was restored to near normal, brain lipopolysaccharide decreased, and catalase activity increased [43]. Caffeic acid and chlorogenic acid have been described as potential antidiabetic agents by increasing glucose uptake in muscle cells. Both compounds were also able to stimulate insulin secretion from an insulin-secreting cell line and islets of Langerhans. Another compound, chicoric acid, is also a new potential antidiabetic agent exhibiting both insulin-sensitizing and insulin-secreting properties [44].

4.6. Gastroprotective Activity

C. intybus has been used in Turkish folklore for its antiulcerogenic potency. The aqueous decoction of C. intybus roots was orally administered to Sprague-Dawley rats 15 minutes before the induction of ulcerogenesis by ethanol. More than 95% inhibition of ulcerogenesis was observed in the test group [45].

4.7. Anti-Inflammatory Activity

The inhibition of TNF-α mediated cyclooxygenase (COX) induction by chicory root extracts was investigated in the human colon carcinoma (HT 29) cell line. The ethyl acetate extract inhibited the production of prostaglandin E2 (PGE2) in a dose-dependent manner. TNF-α mediated induction of COX-2 expression was also suppressed by the chicory extract [46].

4.8. Analgesic Activity

Lactucin, lactucopicrin, and 11β, 13-dihydrolactucin exhibited analgesic action in mice in hot plate and tail-flick tests. In the hot plate test, all three compounds exerted an analgesic effect, with lactucopicrin being the most potent compound. In the tail-flick test, the antinociceptive effects of all the tested compounds (30 mg/kg dose) were comparable to that of ibuprofen (60 mg/kg dose). Lactucin and lactucopicrin were also established to have some sedative action as evident from the decreased spontaneous locomotor activity in mice [47].

4.9. Antioxidant Activity

The DPPH radical scavenging activity of a polyphenols-rich fraction of C. intybus has been investigated [48]. The anti- and prooxidant activities of Cichorium species were studied in chemical as well as biological systems. In the case of chemical systems, the antioxidant activity of water-soluble compounds in C. intybus var. silvestre was established in the coupled model of linoleic acid and β-carotene. A pro-oxidant activity of some of the chemical components was recorded initially which notably diminished with time and/or thermal treatment. Thereafter, the antioxidant activity of the raw juice and its fractions persisted. The molecular weight ranges of the antioxidant fractions of raw juice were also identified based on dialysis [49]. Two varieties of chicory, namely, C. intybus var. silvestre and C. intybus var. foliosum, have been investigated for their antioxidant (antiradical) activities in two distinct biological systems. The lipid peroxidation assay has been carried out on microsome membranes of rat hepatocytes after the induction of oxidative damage by carbon tetrachloride. The antiradical activity was expressed as the protective activity against lipid peroxidation and calculated as the percentage decrease in hydroperoxide degradation products. The second biological system used was the cultures of S. aureus after treatment with cumene hydroperoxide. The percentage increase of growth of bacteria was noted after the treatment with juices of chicory varieties. In both systems, the juices of chicory varieties showed strong antiradical activities [21, 49].

Red chicory (C. intybus var. silvestre) was studied for its polyphenol content and the antioxidant activity was evaluated by using the synthetic 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl radical and three model reactions catalyzed by pertinent enzymatic sources of reactive oxygen species, namely, xanthine oxidase, myeloperoxidase, and diaphorase. Total phenolics were significantly correlated with the antioxidant activity evaluated with both the synthetic radical and the enzyme-catalyzed reactions. On a molar basis, red chicory phenolics were as efficient as Trolox (reference compound) in scavenging the synthetic radical [50]. The aqueous-alcoholic extracts of the aerial parts of C. intybus also inhibited xanthine oxidase enzyme dose dependently [51]. In another study, along with DPPH radical scavenging activity, C. intybus also exhibited inhibition of hydrogen peroxide and chelation of ferrous ion [52].

4.10. Tumor-Inhibitory Activity

The crude ethanolic extract of C. intybus roots caused a significant inhibition of Ehrlich tumor carcinoma in mice. A 70% increase in the life span was observed with a 500 mg/kg/day intraperitoneal dose of the tested extract [53]. The aqueous-alcoholic macerate of the leaves of C. intybus also exerted an antiproliferative effect on amelanotic melanoma C32 cell lines [54]. Magnolialide, a 1β-hydroxyeudesmanolide isolated from the roots of C. intybus, inhibited several tumor cell lines and induced the differentiation of human leukemia HL-60 and U-937 cells to monocyte or macrophage-like cells [55].

4.11. Antiallergic Activity

The aqueous extract of C. intybus inhibited the mast cell-mediated immediate allergic reactions in vitro as well as in vivo. This extract restrained the systemic anaphylactic reaction in mice in a dose-dependent manner. It also significantly inhibited passive cutaneous anaphylactic reaction caused by anti-dinitrophenyl IgE in rats. Other markers of allergic reaction, namely, plasma histamine levels and histamine release from rat peritoneal mast cells, decreased significantly whereas the levels of cAMP increased after the treatment with C. intybus extract [56].

4.12. Other Pharmacologically Important Activities

The ethanol extract of the roots of C. intybus is reported to prevent the immunotoxic effects of ethanol in ICR mice. It was noted that body weight gains were markedly decreased in mice administered with ethanol. However, the body weight was not affected when ethanol was coadministered with the ethanol extract of C. intybus. Similarly, the weights of liver and spleen were not affected when ethanol extract was given along with ethanol. A considerable restoration in the other markers of immunity, namely, hemagglutination titer, plaque forming cells of spleen, secondary IgG antibody production, delayed-type hypersensitivity reaction (in response to subcutaneous administration of sheep red-blood cells to paw), phagocytic activity, number of circulating leucocytes, NK cell activity, cell proliferation, and production of interferon-γ, was registered [57]. The immunoactive potential of an aqueous-alcoholic extract of the roots was established by a mitogen proliferation assay and mixed lymphocyte reaction (MLR). The extract showed an inhibitory effect on lymphocyte proliferation in the presence of phytohemagglutinin and a stimulatory effect on MLR [58].

Chicoric acid has shown vasorelaxant activity against nor-epinephrine-induced contractions in isolated rat aorta strips [59]. A pronounced anticholinesterase activity of the dichloromethane extract of C. intybus roots was seen in the enzyme assay with Ellman’s reagent. Two sesquiterpene lactones, namely, 8-deoxylactucin and lactucopicrin, also exhibited a dose-dependent inhibition of anticholinesterase [60]. The methanolic extract displays wound healing effect and β-sitosterol was determined as the active compound responsible for the activity, possibly due to its significant anti-inflammatory and antioxidant effects, as well as hyaluronidase and collagenase inhibition [7].

5. Toxicological Studies

Although C. intybus has a long history of human use, the high levels of secondary metabolites have shown potential toxicological effects. To evaluate the safety of the root extract of C. intybus, Ames test and subchronic toxicity assessment were conducted. The sesquiterpene-rich extract was evaluated for potential mutagenic properties (Ames test) using Salmonella typhimurium strains TA97a, TA98, TA100, and TA1535 and Escherichia coli strain WP2 uvrA. Though cytotoxicity was observed at high extract doses in some strains, mutagenicity was not noted. A 28-day (subchronic) oral toxicity study, conducted in CRL:CD (SD) IGS BR rats, concluded that there was no extract-related mortality or any other signs of toxicological significance [61]. The toxicity evaluation of C. intybus extracts has also been done by Vibrio fischeri bioluminescence inhibition test (Microtox acute toxicity test). This bacterial test measures the decrease in light emission from the marine luminescent bacteria V. fischeri when exposed to organic extracts. The tested extracts showed less than 20% inhibition of bioluminescence and hence were concluded to be safe for human use [54].

6. Clinical Trials

Two clinical studies on chicory roots are reported in the literature, both of which are pilot studies and are therefore considered to be insufficient to support a well-established use indication for chicory root [5]. The first study, a phase 1, placebo-controlled, double-blind, dose-escalating trial, was conducted to determine the safety and tolerability of a proprietary bioactive extract of chicory root in patients with osteoarthritis (OA) [62]. In general, the treatment was well tolerated. Only one patient who was treated with the highest dose of chicory had to discontinue treatment due to an adverse event. The results of the pilot study suggested that a proprietary bioactive extract of chicory root has a potential role in the management of OA and merits further investigation. The second pilot study was conducted to assess whether chicory coffee consumption might confer cardiovascular benefits; thus, a clinical intervention was performed with 27 healthy volunteers, who consumed 300 mL chicory coffee daily for one week [63]. Depending on the inducer used for the aggregation test, the dietary intervention showed variable effects on platelet aggregation. Whole blood and plasma viscosity were both significantly reduced, along with serum MIF levels, after a week of chicory coffee intake. It was concluded that the full spectrum of the effects was unlikely to be attributed to a single phytochemical; nevertheless, the phenolics (including caffeic acid) are expected to play a substantial role. The study offered an encouraging starting-point to describe the antithrombotic and anti-inflammatory effects of phenolic compounds found in chicory coffee.

In the European Union, there is currently only one registered/authorized herbal medicinal product containing C. intybus as single ingredient whilst there are several combination products on the market [5]. The efficacy of herbal medicine Liv-52 consisting of Mandur bhasma, Tamarix gallica, and herbal extracts of Capparis spinosa, C. intybus, Solanum nigrum, Terminalia arjuna, and Achillea millefolium on liver cirrhosis outcomes was compared with the placebo for 6 months in 36 cirrhotic patients. The study concluded that Liv-52 possessed a hepatoprotective effect in cirrhotic patients. This protective effect of Liv-52 can be attributed to the diuretic, anti-inflammatory, anti-oxidative, and immunomodulating properties of the component herbs [32].

7. Cultivation and Sustainable Use

Greeks and Romans began to grow chicory as a vegetable crop 4000 years ago [9]. Since the discovery in the 1970s that chicory root contains up to 40% inulin (polysaccharide), new strains have been created, with inulin content comparable to that of sugar beet [4]. It is a common vegetable in several Western European countries. It is typically grown in a biennial cycle, with a tuberised root produced during the vegetative growth phase [64]. During the first field year, the vegetative growth phase is characterized by the production of a fleshy taproot. The second field year is the generative phase in which the flowering stem is formed and seeds are produced. To produce the eatable leafy vegetable called a chicon, roots are harvested at the end of the first growing period when an appropriate stage of maturity is reached [65]. The application of inulin in the food industry was restricted to the production of coffee substitutes. It was later discovered that inulin could act as a substitute for sugar or fat due to its low caloric value. The most stable form for the commercialization of inulin is the powdered extract for its greater facility of manipulation, transport, storage, and consumption [66].

Chicory is especially attractive as a cash crop since it can reach more than 62 t ha−1 under favourable conditions. Inulin content can reach on average 15% of root fresh weight and a yield of 8 t ha−1 of inulin is achievable [67]. The USA imports more than 2.3 million kilograms of chicons and 1.9 million kilograms of roasted chicory roots for coffee according to 2002 US Department of Commerce tariff and trade data [61]. Numerous studies have focused on different cultivation aspects of chicory. Chicory is considered one of the most important sources of inulin since it has a high root yield potential and also a high root sugar content [68]. A high root yield, a high inulin content, and especially long inulin chains are preferred [69]. Short chain inulin is used for the production of fructose syrup used in sweetening of cold drinks whereas long chain inulin is used as fat replacer and foam stabilizer in food products and also in the production of carboxymethyl inulin [2]. The effect of fertilizers on the growth, development, and yield of chicory has been well studied. In general, increase of nitrogen (N) increases the growth and ultimately the yield, although a high application of N has a negative effect on especially some of the amino acids. Increased N application at levels of 200 kg N ha−1 leads to a decrease in amino acids such as threonine and valine with a pronounced decreased effect on methionine. A level of 100 kg N ha−1 is preferred for enhanced quality [70]. In terms of phosphorous (P), it has been established that chicory has at least two inherent patterns of response to low or zero P conditions. One pattern is the classical increase in the length of the smallest diameter roots in response to P deficient conditions. The second pattern is a significant decrease in root tissue density under low P conditions [71]. It has also been investigated as a suitable catch crop since it has the ability to withdraw N, especially nitrate, from the soil, thereby reducing potential leaching. Additionally, it can withstand competition, has a slow juvenile development and vigorous growth after harvest of the main crop, is frost- and winter-hardy, has a well-developed root system, and does not transmit pathogens or pests to other crops [72]. Canopy closure in chicory is advantageous and critical for yield and can be achieved by a larger supply of assimilates to the shoot [73].

Chicory is greatly influenced by the pH of the soil as this affects the availability of nutrients in the soil to the plant. A study conducted by Anguissola Scotti el al. [74] on two soils of pH, 5.7 and 7.0 indicated that the fly-ash or metal availability to the plant was significantly different at the different pH levels. At a low pH a decrease of Zn, Cu, Cd, and Ni was observed and for neutral soils the added metals are more available to plants than those naturally occurring in soils [75]. Chicory is a cold-requiring long-day plant [72]. In a study by Amaducci and Pritoni [68], it was shown that retarding harvest time significantly affects the content and concentration of inulin. Rainfall has proven to be another important factor since the roots contain a higher water content, thereby affecting the concentration of inulin in the roots. The same results have been obtained in a study by Baert [69] where an early sowing date and harvesting time increased the root yield, total sugar content, and inulin chain length. The increase in yield with an earlier sowing date was up to 30% higher with an increase of 10% with an earlier harvest date. The content of free fructose and sucrose increased and the content of free glucose and inulin decreased with a later harvest time [75].

Even though chicory is a cold-requiring plant, cold storage after harvest can cause a strong decrease in free glucose, an increase in free fructose and sucrose, and hydrolysis of inulin [76]. This has also been observed by Ernst et al. [77] where an onset of cooler temperatures and especially colder temperatures during storage resulted in an increase in sucrose and fructose content. It was found that sucrose increased in the roots of chicory about threefold and fructose increased about tenfold within the first few weeks of cold storage after harvest [77]. The effect of storage at reduced temperature has also been studied on the levels of sesquiterpene lactones. Storage at 2°C and 10°C for up to 13 days had no effect on the level of lactucin-like sesquiterpene lactones in the chicons and after 7 days of storage a slight increase of lactucopicrin content was observed [78]. Several types of discoloration, leaf edge damage, and extensive growth of the internal core can occur in the heads of chicory during postharvest storage, which considerably reduces their market value. An atmospheric composition of 10% O2 and 10% CO2 in combination with a storage temperature of 5°C was found optimal [64]. Low temperature in the field also hastens and enhances bolting and flowering [79, 80]. The type and cultivar have been identified in numerous studies to be the determining factor during the stages of growth [81]. Suhonen [82] found the highest numbers of bolters in those being planted last, for which the mean temperatures during early growth were the highest.

During the postharvest period, the major polyamines present are putrescine, especially in the oldest leaves, although spermidine is present in considerable amounts, showing a tendency to decrease with the increasing physiological age of the leaves. Free sterol content increases with postharvest and also with physiological age of the leaves. Sitosterol is the major free sterol present, followed by stigmasterol and campesterol [83].

Emergence has been reported as one negative aspect of the crop, which could be addressed by specific breeding programmes [68]. Chicory can also be propagated and grown by means of micropropagation by regeneration of meristematic nodules. The leaves are cultivated in vitro and develop into plantlets when transferred to soil [84]. Although chicory can be regenerated in vitro from explants, both through organogenesis and somatic embryogenesis, and from protoplasts, no transgenic plants have yet been reported that have been produced [85].

Promising potential utility technologies of the plant have emerged. Chicory roots pulps are an important by-product of the inulin processing industries and are usually used in animal feed. Other applications can be found for these materials since the extraction of chicory pulp yields high levels of pectin, a polysaccharide extensively used in food as a gelling agent, thickening agent, and stabilizer [86]. The crude protein content in chicory is more valuable than in alfalfa. Furthermore the crude lipid in chicory is generally higher than most varieties of alfalfa [9]. It also has a nutritional quality comparable to lucerne, with a mineral content superior for copper and zinc, with similar proportions of protein, lipid, minerals, and other nutrients, and palatable with good digestion with applicability in the poultry and livestock industry [87, 88].

8. Conclusion

Cichorium intybus has a long tradition of use globally. Historically, chicory was grown by the ancient Egyptians as a medicinal plant, coffee substitute, and vegetable crop and was occasionally used for animal forage. This multipurpose plant contains high amounts of proteins, carbohydrates, and mineral elements [9]. Inulin from chicory roots is considered a functional food ingredient as it affects physiological and biochemical processes resulting in better health and reduction of the risk of many diseases [89].

To date, chicory remains an extremely versatile plant, amenable to genetic manipulation, and there is interest shown in genetically engineered chicory to obtain higher yields and create new potentials [1]. The documented indigenous knowledge relating to the various medicinal uses of chicory has been supported by phytochemical isolation and investigations into biological activity. Nonetheless, many of its constituents have not been explored for their pharmacological potential and further research is necessary to gain better understanding of the phytochemicals against various diseases [9]. Toxicological data on C. intybus is currently limited; however, considering that the Asteraceae family is a known source of allergic problems, a contraindication for hypersensitivity should be included in the safety data [5]. Recent studies suggest the use of C. intybus as a biomonitor for heavy metals [90, 91]; considering that chicory enters the food chain, this plant should be used with caution. The apparent bioactivity of C. intybus shown in preclinical studies (both in vitro and in vivo) is a testament to its historical use in traditional medicine.

Conflict of Interests

The authors declare no conflict of interests.

Acknowledgments

The South African Medical Research Council, University of South Africa and the HOD, School of Pharmaceutical Sciences, Lovely Professional University, Punjab, India, are thanked for their support.

References

  1. H. P. Bais and G. A. Ravishankar, “Cichorium intybus L.—cultivation, processing, utility, value addition and biotechnology, with an emphasis on current status and future prospects,” Journal of the Science of Food and Agriculture, vol. 81, no. 5, pp. 467–484, 2001. View at Publisher · View at Google Scholar · View at Scopus
  2. J. van Arkel, R. Vergauwen, R. Sévenier et al., “Sink filling, inulin metabolizing enzymes and carbohydrate status in field grown chicory (Cichorium intybus L.),” Journal of Plant Physiology, vol. 169, no. 15, pp. 1520–1529, 2012. View at Google Scholar
  3. B. E. van Wyk, B. van Oudtshoorn, and N. Gericke, Medicinal Plants of South Africa, Briza Publications, Pretoria, South Africa, 1997.
  4. A. Judžentienė and J. Būdienė, “Volatile constituents from aerial parts and roots of Cichorium intybus L. (chicory) grown in Lithuania,” Chemija, vol. 19, pp. 25–28, 2008. View at Google Scholar
  5. European Medicines Agency, “Assessment report on Cichorium intybus L., radix,” EMA/HMPC/113041/2010, 2013.
  6. T. Cadalen, M. Mörchen, C. Blassiau et al., “Development of SSR markers and construction of a consensus genetic map for chicory (Cichorium intybus L.),” Molecular Breeding, vol. 25, no. 4, pp. 699–722, 2010. View at Publisher · View at Google Scholar · View at Scopus
  7. I. Süntar, E. K. Akkola, H. Kelesb, E. Yesiladac, S. D. Sarkerd, and T. Baykala, “Comparative evaluation of traditional prescriptions from Cichorium intybus L. for wound healing: stepwise isolation of an active component by in vivo bioassay and its mode of activity,” Journal of Ethnopharmacology, vol. 143, no. 1, pp. 299–309, 2012. View at Publisher · View at Google Scholar
  8. A. Gurib-Fakim, “Medicinal plants: traditions of yesterday and drugs of tomorrow,” Molecular Aspects of Medicine, vol. 27, no. 1, pp. 1–93, 2006. View at Publisher · View at Google Scholar · View at Scopus
  9. Q. Wang and J. Cui, “Perspectives and utilization technologies of chicory (Cichorium intybus L.): a review,” African Journal of Biotechnology, vol. 10, no. 11, pp. 1966–1977, 2011. View at Google Scholar · View at Scopus
  10. E. Sezik, E. Yeşilada, G. Honda, Y. Takaishi, Y. Takeda, and T. Tanaka, “Traditional medicine in Turkey X. Folk medicine in Central Anatolia,” Journal of Ethnopharmacology, vol. 75, no. 2-3, pp. 95–115, 2001. View at Publisher · View at Google Scholar · View at Scopus
  11. European Medicines Agency, “Community herbal monograph on Cichorium intybus L., radix,” EMA/HMPC/121816/2010, 2012.
  12. T. A. Bischoff, C. J. Kelley, Y. Karchesy, M. Laurantos, P. Nguyen-Dinh, and A. G. Arefi, “Antimalarial activity of Lactucin and Lactucopicrin: sesquiterpene lactones isolated from Cichorium intybus L.,” Journal of Ethnopharmacology, vol. 95, no. 2-3, pp. 455–457, 2004. View at Publisher · View at Google Scholar · View at Scopus
  13. A. Pieroni, “Medicinal plants and food medicines in the folk traditions of the upper Lucca Province, Italy,” Journal of Ethnopharmacology, vol. 70, no. 3, pp. 235–273, 2000. View at Publisher · View at Google Scholar · View at Scopus
  14. B. Ahmed, T. A. Al-Howiriny, and A. B. Siddiqui, “Antihepatotoxic activity of seeds of Cichorium intybus,” Journal of Ethnopharmacology, vol. 87, no. 2-3, pp. 237–240, 2003. View at Publisher · View at Google Scholar · View at Scopus
  15. E. Hanlidou, R. Karousou, V. Kleftoyanni, and S. Kokkini, “The herbal market of Thessaloniki (N Greece) and its relation to the ethnobotanical tradition,” Journal of Ethnopharmacology, vol. 91, no. 2-3, pp. 281–299, 2004. View at Publisher · View at Google Scholar · View at Scopus
  16. S. Jarić, Z. Popović, M. Mačukanović-Jocić et al., “An ethnobotanical study on the usage of wild medicinal herbs from Kopaonik Mountain (Central Serbia),” Journal of Ethnopharmacology, vol. 111, no. 1, pp. 160–175, 2007. View at Publisher · View at Google Scholar · View at Scopus
  17. P. N. Pushparaj, H. K. Low, J. Manikandan, B. K. H. Tan, and C. H. Tan, “Anti-diabetic effects of Cichorium intybus in streptozotocin-induced diabetic rats,” Journal of Ethnopharmacology, vol. 111, no. 2, pp. 430–434, 2007. View at Publisher · View at Google Scholar · View at Scopus
  18. C. Carazzone, D. Mascherpa, G. Gazzani, and A. Papetti, “Identification of phenolic constituents in red chicory salads (Cichorium intybus) by high-performance liquid chromatography with diode array detection and electrospray ionisation tandem mass spectrometry,” Food Chemistry, vol. 138, pp. 1062–1071, 2013. View at Publisher · View at Google Scholar
  19. R. Norbak, K. Nielsen, and T. Kondo, “Anthocyanins from flowers of Cichorium intybus,” Phytochemistry, vol. 60, no. 4, pp. 357–359, 2002. View at Publisher · View at Google Scholar · View at Scopus
  20. B. Suresh, P. D. Sherkhane, S. Kale, S. Eapen, and G. A. Ravishankar, “Uptake and degradation of DDT by hairy root cultures of Cichorium intybus and Brassica juncea,” Chemosphere, vol. 61, no. 9, pp. 1288–1292, 2005. View at Publisher · View at Google Scholar · View at Scopus
  21. G. Gazzani, M. Daglia, A. Papetti, and C. Gregotti, “In vitro and ex vivo anti- and prooxidant components of Cichorium intybus,” Journal of Pharmaceutical and Biomedical Analysis, vol. 23, no. 1, pp. 127–133, 2000. View at Publisher · View at Google Scholar · View at Scopus
  22. T. Shaikh, R. A. Rub, and S. Sasikumar, “Antimicrobial screening of Cichorium intybus seed extracts,” Arabian Journal of Chemistry, 2012. View at Publisher · View at Google Scholar
  23. S. Nandagopal and R. B. D. Kumari, “Phytochemical and antibacterial studies of chicory (Cichorium intybus L.)—a multipurpose medicinal plant,” Advances in Biological Research, vol. 1, no. 1-2, pp. 17–21, 2007. View at Google Scholar
  24. P. Rani and N. Khullar, “Antimicrobial evaluation of some medicinal plants for their anti-enteric potential against multi-drug resistant Salmonella typhi,” Phytotherapy Research, vol. 18, no. 8, pp. 670–673, 2004. View at Publisher · View at Google Scholar · View at Scopus
  25. D. Mares, C. Romagnoli, B. Tosi, E. Andreotti, G. Chillemi, and F. Poli, “Chicory extracts from Cichorium intybus L. as potential antifungals,” Mycopathologia, vol. 160, no. 1, pp. 85–91, 2005. View at Publisher · View at Google Scholar · View at Scopus
  26. K. Monde, T. Oya, A. Shirata, and M. Takasugi, “A guaianolide phytoalexin, cichoralexin, from Cichorium intybus,” Phytochemistry, vol. 29, no. 11, pp. 3449–3451, 1990. View at Google Scholar · View at Scopus
  27. M. C. Miller, S. K. Duckett, and J. G. Andrae, “The effect of forage species on performance and gastrointestinal nematode infection in lambs,” Small Ruminant Research, vol. 95, no. 2-3, pp. 188–192, 2011. View at Publisher · View at Google Scholar · View at Scopus
  28. C. L. Marley, R. Cook, R. Keatinge, J. Barrett, and N. H. Lampkin, “The effect of birdsfoot trefoil (Lotus corniculatus) and chicory (Cichorium intybus) on parasite intensities and performance of lambs naturally infected with helminth parasites,” Veterinary Parasitology, vol. 112, no. 1-2, pp. 147–155, 2003. View at Publisher · View at Google Scholar · View at Scopus
  29. A. L. Molan, A. J. Duncan, T. N. Barry, and W. C. McNabb, “Effects of condensed tannins and crude sesquiterpene lactones extracted from chicory on the motility of larvae of deer lungworm and gastrointestinal nematodes,” Parasitology International, vol. 52, no. 3, pp. 209–218, 2003. View at Publisher · View at Google Scholar · View at Scopus
  30. J. G. Foster, K. A. Cassida, and K. E. Turner, “In vitro analysis of the anthelmintic activity of forage chicory (Cichorium intybus L.) sesquiterpene lactones against a predominantly Haemonchus contortus egg population,” Veterinary Parasitology, vol. 180, no. 3-4, pp. 298–306, 2011. View at Publisher · View at Google Scholar · View at Scopus
  31. E. Leclercq, “Determination of lactucin in roots of chicory (Cichorium intybus L.) by high-performance liquid chromatography,” Journal of Chromatography A, vol. 283, pp. 441–444, 1984. View at Google Scholar · View at Scopus
  32. H. Fallah Huseini, S. M. Alavian, R. Heshmat, M. R. Heydari, and K. Abolmaali, “The efficacy of Liv-52 on liver cirrhotic patients: a randomized, double-blind, placebo-controlled first approach,” Phytomedicine, vol. 12, no. 9, pp. 619–624, 2005. View at Publisher · View at Google Scholar · View at Scopus
  33. A. K. Najmi, K. K. Pillai, S. N. Pal, and M. Aqil, “Free radical scavenging and hepatoprotective activity of jigrine against galactosamine induced hepatopathy in rats,” Journal of Ethnopharmacology, vol. 97, no. 3, pp. 521–525, 2005. View at Publisher · View at Google Scholar · View at Scopus
  34. A. H. Gilani and K. H. Janbaz, “Evaluation of the liver protective potential of Cichorium intybus seed extract on acetaminophen and CC14-induced damage,” Phytomedicine, vol. 1, no. 3, pp. 193–197, 1994. View at Google Scholar · View at Scopus
  35. R. Zafar and S. Mujahid Ali, “Anti-hepatotoxic effects of root and root callus extracts of Cichorium intybus L.,” Journal of Ethnopharmacology, vol. 63, no. 3, pp. 227–231, 1998. View at Publisher · View at Google Scholar · View at Scopus
  36. A. H. Gilani, K. H. Janbaz, and B. H. Shah, “Esculetin prevents liver damage induced by paracetamol and CCL4,” Pharmacological Research, vol. 37, no. 1, pp. 31–35, 1998. View at Publisher · View at Google Scholar · View at Scopus
  37. B. Ahmed, S. Khan, M. H. Masood, and A. H. Siddique, “Anti-hepatotoxic activity of cichotyboside, a sesquiterpene glycoside from the seeds of Cichorium intybus,” Journal of Asian Natural Products Research, vol. 10, no. 3-4, pp. 223–231, 2008. View at Google Scholar · View at Scopus
  38. C. Gadgoli and S. H. Mishra, “Antihepatotoxic activity of Cichorium intybus,” Journal of Ethnopharmacology, vol. 58, no. 2, pp. 131–134, 1997. View at Publisher · View at Google Scholar · View at Scopus
  39. N. Ziamajidi, S. Khaghania, G. Hassanzadeh et al., “Amelioration by chicory seed extract of diabetes- and oleic acid-induced non-alcoholic fatty liver disease (NAFLD)/non-alcoholic steatohepatitis (NASH) via modulation of PPAR-alpha and SREBP-1,” Food and Chemical Toxicology, vol. 58, pp. 198–209, 2013. View at Publisher · View at Google Scholar
  40. S. Sultana, S. Perwaiz, M. Iqbal, and M. Athar, “Crude extracts of hepatoprotective plants, solanum nigrum and cichoriunz intybus inhibit free radical-mediated DNA damage,” Journal of Ethnopharmacology, vol. 45, no. 3, pp. 189–192, 1995. View at Publisher · View at Google Scholar · View at Scopus
  41. F. M. Hardeep and D. K. Pandey, “Anti-diabetic activity of methanolic extract of chicory roots in streptozocin induced diabetic rats,” International Journal of Pharmacy, vol. 3, no. 1, pp. 211–216, 2013. View at Google Scholar
  42. A. Ghamarian, M. Abdollahi, X. Su, A. Amiri, A. Ahadi, and A. Nowrouzi, “Effect of chicory seed extract on glucose tolerance test (GTT) and metabolic profile in early and late stage diabetic rats,” DARU Journal of Pharmaceutical Sciences, vol. 20, pp. 56–65, 2012. View at Publisher · View at Google Scholar
  43. M. Ahmad, R. Qureshi, M. Arshad, M. A. Khan, and M. Zafar, “Traditional herbal remedies used for the treatment of diabetes from district attock (Pakistan),” Pakistan Journal of Botany, vol. 41, no. 6, pp. 2777–2782, 2009. View at Google Scholar · View at Scopus
  44. D. Tousch, A.-D. Lajoix, E. Hosy et al., “Chicoric acid, a new compound able to enhance insulin release and glucose uptake,” Biochemical and Biophysical Research Communications, vol. 377, no. 1, pp. 131–135, 2008. View at Publisher · View at Google Scholar · View at Scopus
  45. I. Gürbüz, O. Üstün, E. Yeşilada, E. Sezik, and N. Akyürek, “In vivo gastroprotective effects of five Turkish folk remedies against ethanol-induced lesions,” Journal of Ethnopharmacology, vol. 83, no. 3, pp. 241–244, 2002. View at Publisher · View at Google Scholar · View at Scopus
  46. C. Cavin, M. Delannoy, A. Malnoe et al., “Inhibition of the expression and activity of cyclooxygenase-2 by chicory extract,” Biochemical and Biophysical Research Communications, vol. 327, no. 3, pp. 742–749, 2005. View at Publisher · View at Google Scholar · View at Scopus
  47. A. Wesołowska, A. Nikiforuk, K. Michalska, W. Kisiel, and E. Chojnacka-Wójcik, “Analgesic and sedative activities of lactucin and some lactucin-like guaianolides in mice,” Journal of Ethnopharmacology, vol. 107, no. 2, pp. 254–258, 2006. View at Publisher · View at Google Scholar · View at Scopus
  48. D. Heimler, L. Isolani, P. Vignolini, and A. Romani, “Polyphenol content and antiradical activity of Cichorium intybus L. from biodynamic and conventional farming,” Food Chemistry, vol. 114, no. 3, pp. 765–770, 2009. View at Publisher · View at Google Scholar · View at Scopus
  49. A. Papetti, M. Daglia, P. Grisoli, C. Dacarro, C. Gregotti, and G. Gazzani, “Anti- and pro-oxidant activity of Cichorium genus vegetables and effect of thermal treatment in biological systems,” Food Chemistry, vol. 97, no. 1, pp. 157–165, 2006. View at Publisher · View at Google Scholar · View at Scopus
  50. V. Lavelli, “Antioxidant activity of minimally processed red chicory (Cichorium intybus L.) evaluated in xanthine oxidase-, myeloperoxidase-, and diaphorase-catalyzed reactions,” Journal of Agricultural and Food Chemistry, vol. 56, no. 16, pp. 7194–7200, 2008. View at Publisher · View at Google Scholar · View at Scopus
  51. A. Pieroni, V. Janiak, C. M. Dürr, S. Lüdeke, E. Trachsel, and M. Heinrich, “In vitro antioxidant activity of non-cultivated vegetables of ethnic Albanians in southern Italy,” Phytotherapy Research, vol. 16, no. 5, pp. 467–473, 2002. View at Publisher · View at Google Scholar · View at Scopus
  52. S. N. El and S. Karakaya, “Radical scavenging and iron-chelating activities of some greens used as traditional dishes in Mediterranean diet,” International Journal of Food Sciences and Nutrition, vol. 55, no. 1, pp. 67–74, 2004. View at Publisher · View at Google Scholar · View at Scopus
  53. B. Hazra, R. Sarkar, S. Bhattacharyya, and P. Roy, “Tumour inhibitory activity of chicory root extract against Ehrlich ascites carcinoma in mice,” Fitoterapia, vol. 73, no. 7-8, pp. 730–733, 2002. View at Publisher · View at Google Scholar · View at Scopus
  54. F. Conforti, G. Ioele, G. A. Statti, M. Marrelli, G. Ragno, and F. Menichini, “Antiproliferative activity against human tumor cell lines and toxicity test on Mediterranean dietary plants,” Food and Chemical Toxicology, vol. 46, no. 10, pp. 3325–3332, 2008. View at Publisher · View at Google Scholar · View at Scopus
  55. K.-T. Lee, J.-I. Kim, H.-J. Park, K.-O. Yoo, Y.-N. Han, and K.-I. Miyamoto, “Differentiation-inducing effect of magnolialide, a 1β-hydroxyeudesmanolide isolated from Cichorium intybus, on human leukemia cells,” Biological and Pharmaceutical Bulletin, vol. 23, no. 8, pp. 1005–1007, 2000. View at Google Scholar · View at Scopus
  56. H. M. Kim, H. W. Kim, Y. S. Lyu et al., “Inhibitory effect of mast cell-mediated immediate-type allergic reactions by Cichorium intybus,” Pharmacological Research, vol. 40, no. 1, pp. 61–65, 1999. View at Publisher · View at Google Scholar · View at Scopus
  57. J.-H. Kim, Y.-J. Mun, W.-H. Woo, K.-S. Jeon, N.-H. An, and J.-S. Park, “Effects of the ethanol extract of Cichorium intybus on the immunotoxicity by ethanol in mice,” International Immunopharmacology, vol. 2, no. 6, pp. 733–744, 2002. View at Publisher · View at Google Scholar · View at Scopus
  58. Z. Amirghofran, M. Azadbakht, and M. H. Karimi, “Evaluation of the immunomodulatory effects of five herbal plants,” Journal of Ethnopharmacology, vol. 72, no. 1-2, pp. 167–172, 2000. View at Publisher · View at Google Scholar · View at Scopus
  59. N. Sakurai, T. Iizuka, S. Nakayama, H. Funayama, M. Noguchi, and M. Nagai, “Vasorelaxant activity of caffeic acid derivatives from Cichorium intybus and Equisetum arvense,” Yakugaku Zasshi, vol. 123, no. 7, pp. 593–598, 2003. View at Google Scholar · View at Scopus
  60. J. M. Rollinger, P. Mock, C. Zidorn, E. P. Ellmerer, T. Langer, and H. Stuppner, “Application of the in combo screening approach for the discovery of non-alkaloid acetylcholinesterase inhibitors from Cichorium intybus,” Current Drug Discovery Technologies, vol. 2, no. 3, pp. 185–193, 2005. View at Publisher · View at Google Scholar · View at Scopus
  61. B. M. Schmidt, N. Ilic, A. Poulev, and I. Raskin, “Toxicological evaluation of a chicory root extract,” Food and Chemical Toxicology, vol. 45, no. 7, pp. 1131–1139, 2007. View at Publisher · View at Google Scholar · View at Scopus
  62. N. J. Olsen, V. K. Branch, G. Jonnala, M. Seskar, and M. Cooper, “Phase 1, placebo-controlled, dose escalation trial of chicory root extract in patients with osteoarthritis of the hip or knee,” BMC Musculoskeletal Disorders, vol. 11, article 156, no. 1, 2010. View at Publisher · View at Google Scholar · View at Scopus
  63. E. Schumacher, É. Vigh, V. Molnár et al., “Thrombosis preventive potential of chicory coffee consumption: a clinical study,” Phytotherapy Research, vol. 25, no. 5, pp. 744–748, 2011. View at Publisher · View at Google Scholar · View at Scopus
  64. E. Vanstreels, J. Lammertyn, B. E. Verlinden, N. Gillis, A. Schenk, and B. M. Nicolaï, “Red discoloration of chicory under controlled atmosphere conditions,” Postharvest Biology and Technology, vol. 26, no. 3, pp. 313–322, 2002. View at Publisher · View at Google Scholar · View at Scopus
  65. I. M. François, E. Mariën, K. Brijs, P. Coppin, and M. de Proft, “The use of Vis/NIR spectroscopy to predict the optimal root harvesting date of chicory (Cichorium intybus L.),” Postharvest Biology and Technology, vol. 53, no. 1-2, pp. 77–83, 2009. View at Publisher · View at Google Scholar · View at Scopus
  66. G. M. Figueira, K. J. Park, F. P. R. Brod, and S. L. Honório, “Evaluation of desorption isotherms, drying rates and inulin concentration of chicory roots (Cichorium intybus L.) with and without enzymatic inactivation,” Journal of Food Engineering, vol. 63, no. 3, pp. 273–280, 2004. View at Publisher · View at Google Scholar · View at Scopus
  67. A. Papetti, D. Mascherpaa, C. Carazzonea et al., “Identification of organic acids in Cichorium intybus inhibiting virulence-related properties of oral pathogenic bacteria,” Food Chemistry, vol. 138, no. 2-3, pp. 1706–1712, 2013. View at Publisher · View at Google Scholar
  68. S. Amaducci and G. Pritoni, “Effect of harvest date and cultivar on Cichorium intybus yield components in north Italy,” Industrial Crops and Products, vol. 7, no. 2-3, pp. 345–349, 1998. View at Publisher · View at Google Scholar · View at Scopus
  69. J. R. A. Baert, “The effect of sowing and harvest date and cultivar on inulin yield and composition of chicory (Cichorium intybus L.) roots,” Industrial Crops and Products, vol. 6, no. 3-4, pp. 195–199, 1997. View at Publisher · View at Google Scholar · View at Scopus
  70. M. Ćustić, M. Horvatić, and A. Butorac, “Effects of nitrogen fertilization upon the content of essential amino acids in head chicory (Cichorium intybus L. var. foliosum),” Scientia Horticulturae, vol. 92, no. 3-4, pp. 205–215, 2002. View at Publisher · View at Google Scholar · View at Scopus
  71. R. W. Zobel, G. A. Alloush, and D. P. Belesky, “Differential root morphology response to no versus high phosphorus, in three hydroponically grown forage chicory cultivars,” Environmental and Experimental Botany, vol. 57, no. 1-2, pp. 201–208, 2006. View at Publisher · View at Google Scholar · View at Scopus
  72. E. Zagal, I. Rydberg, and A. Mårtensson, “Carbon distribution and variations in nitrogen-uptake between catch crop species in pot experiments,” Soil Biology and Biochemistry, vol. 33, no. 4-5, pp. 523–532, 2001. View at Publisher · View at Google Scholar · View at Scopus
  73. W. J. M. Meijer and E. W. J. M. Mathijssen, “Experimental and simulated production of inulin by chicory and Jerusalem artichoke,” Industrial Crops and Products, vol. 1, no. 2–4, pp. 175–183, 1992. View at Google Scholar · View at Scopus
  74. I. Anguissola Scotti, S. Silva, and G. Botteschi, “Effect of fly ash on the availability of Zn, Cu, Ni and Cd to chicory,” Agriculture, Ecosystems & Environment, vol. 72, no. 2, pp. 159–163, 1999. View at Publisher · View at Google Scholar · View at Scopus
  75. G. Varallo, G. Membola, and G. DellAgnola, “Cropping of fly ash-amended soils: effects on soil chemical properties and yield and elemental composition of Cichorium intybus L.,” Fuel, vol. 72, no. 5, p. 725, 1993. View at Google Scholar
  76. M. A. C. Demeulemeester, V. Verdoodt, and M. P. de Proft, “Interaction between physiological age and cold treatment on the composition and concentration of carbohydrates in chicory roots (Cichorium intybus L.),” Journal of Plant Physiology, vol. 153, no. 3-4, pp. 467–475, 1998. View at Google Scholar · View at Scopus
  77. M. Ernst, N. J. Chatterton, and P. A. Harrison, “Carbohydrate changes in chicory (Cichorium intybus L. var. foliosum) during growth and storage,” Scientia Horticulturae, vol. 63, no. 3-4, pp. 251–261, 1995. View at Publisher · View at Google Scholar · View at Scopus
  78. A. M. Peters and A. van Amerongen, “Sesquiterpene lactones in chicory (Cichorium intybus L.): distribution in chicons and effect of storage,” Food Research International, vol. 29, no. 5-6, pp. 439–444, 1996. View at Google Scholar · View at Scopus
  79. G. Gianquinto, “Morphological and physiological aspects of phase transition in radicchio (Cichorium intybus L. var. silvestre Bisch.): influence of daylength and its interaction with low temperature,” Scientia Horticulturae, vol. 71, no. 1-2, pp. 13–26, 1997. View at Publisher · View at Google Scholar · View at Scopus
  80. H.-J. Wiebe, “Effects of low temperature during seed development on the mother plant on subsequent bolting of chicory, lettuce and spinach,” Scientia Horticulturae, vol. 38, no. 3-4, pp. 223–229, 1989. View at Google Scholar · View at Scopus
  81. F. Poli, G. Sacchetti, B. Tosi et al., “Variation in the content of the main guaianolides and sugars in Cichorium intybus var. “Rosso di Chioggia” selections during cultivation,” Food Chemistry, vol. 76, no. 2, pp. 139–147, 2002. View at Publisher · View at Google Scholar · View at Scopus
  82. I. Suhonen, “Growth, bolting and yield quality of ‘radicchio rosso’,” Scientia Horticulturae, vol. 46, no. 1-2, pp. 25–31, 1991. View at Google Scholar · View at Scopus
  83. E. O. Krebsky, J. M. C. Geuns, and M. de Proft, “Polyamines and sterois in Cichorium heads,” Phytochemistry, vol. 50, no. 4, pp. 549–553, 1999. View at Publisher · View at Google Scholar · View at Scopus
  84. S. Piéron, M. Belaizi, and P. Boxus, “Nodule culture, a possible morphogenetic pathway in Cichorium intybus L. propagation,” Scientia Horticulturae, vol. 53, no. 1-2, pp. 1–11, 1993. View at Google Scholar · View at Scopus
  85. J. Wijbrandi and M. T. J. de Both, “Temperate vegetable crops,” Scientia Horticulturae, vol. 55, no. 1-2, pp. 37–63, 1993. View at Google Scholar · View at Scopus
  86. C. Robert, T. H. Emaga, B. Wathelet, and M. Paquot, “Effect of variety and harvest date on pectin extracted from chicory roots (Cichorium intybus L.),” Food Chemistry, vol. 108, no. 3, pp. 1008–1018, 2008. View at Publisher · View at Google Scholar · View at Scopus
  87. M. A. Sanderson, M. Labreveux, M. H. Hall, and G. F. Elwinger, “Nutritive value of chicory and English plantain forage,” Crop Science, vol. 43, no. 5, pp. 1797–1804, 2003. View at Google Scholar · View at Scopus
  88. A. Scharenberg, Y. Arrigo, A. Gutzwiller et al., “Palatability in sheep and in vitro nutritional value of dried and ensiled sainfoin (Onobrychis viciifolia) birdsfoot trefoil (Lotus corniculatus), and chicory (Cichorium intybus),” Archives of Animal Nutrition, vol. 61, no. 6, pp. 481–496, 2007. View at Publisher · View at Google Scholar · View at Scopus
  89. N. Kaur and A. K. Gupta, “Applications of inulin and oligofructose in health and nutrition,” Journal of Biosciences, vol. 27, no. 7, pp. 703–714, 2002. View at Google Scholar · View at Scopus
  90. A. Aksoy, “Chicory (Cichorium intybus L.): a possible biomonitor of metal pollution,” Pakistan Journal of Botany, vol. 40, no. 2, pp. 791–797, 2008. View at Google Scholar · View at Scopus
  91. D. Yakupoǧlu, T. Güray, D. Yurtsever Sarica, and Z. Kaya, “Determination of airborne lead contamination in Cichorium intybus L. in an urban environment,” Turkish Journal of Botany, vol. 32, no. 4, pp. 319–324, 2008. View at Google Scholar · View at Scopus
  92. B. Šarić-Kundalić, C. Dobeš, V. Klatte-Asselmeyer, and J. Saukel, “Ethnobotanical survey of traditionally used plants in human therapy of east, north and north-east Bosnia and Herzegovina,” Journal of Ethnopharmacology, vol. 133, no. 3, pp. 1051–1076, 2011. View at Publisher · View at Google Scholar · View at Scopus
  93. M. L. Leporatti and S. Ivancheva, “Preliminary comparative analysis of medicinal plants used in the traditional medicine of Bulgaria and Italy,” Journal of Ethnopharmacology, vol. 87, no. 2-3, pp. 123–142, 2003. View at Publisher · View at Google Scholar · View at Scopus
  94. E. Miraldi, S. Ferri, and V. Mostaghimi, “Botanical drugs and preparations in the traditional medicine of West Azerbaijan (Iran),” Journal of Ethnopharmacology, vol. 75, no. 2-3, pp. 77–87, 2001. View at Publisher · View at Google Scholar · View at Scopus
  95. P. M. Guarrera, G. Forti, and S. Marignoli, “Ethnobotanical and ethnomedicinal uses of plants in the district of Acquapendente (Latium, Central Italy),” Journal of Ethnopharmacology, vol. 96, no. 3, pp. 429–444, 2005. View at Publisher · View at Google Scholar · View at Scopus
  96. M. C. Loi, L. Maxia, and A. Maxia, “Ethnobotanical comparison between the villages of Escolca and Lotzorai (Sardinia, Italy),” Journal of Herbs, Spices & Medicinal Plants, vol. 11, no. 3, pp. 67–84, 2005. View at Publisher · View at Google Scholar · View at Scopus
  97. A. Pieroni, C. Quave, S. Nebel, and M. Heinrich, “Ethnopharmacy of the ethnic Albanians (Arbëreshë) of northern Basilicata, Italy,” Fitoterapia, vol. 73, no. 3, pp. 217–241, 2002. View at Publisher · View at Google Scholar · View at Scopus
  98. H. Jouad, M. Haloui, H. Rhiouani, J. El Hilaly, and M. Eddouks, “Ethnobotanical survey of medicinal plants used for the treatment of diabetes, cardiac and renal diseases in the North centre region of Morocco (Fez-Boulemane),” Journal of Ethnopharmacology, vol. 77, no. 2-3, pp. 175–182, 2001. View at Publisher · View at Google Scholar · View at Scopus
  99. J. El-Hilaly, M. Hmammouchi, and B. Lyoussi, “Ethnobotanical studies and economic evaluation of medicinal plants in Taounate province (Northern Morocco),” Journal of Ethnopharmacology, vol. 86, no. 2-3, pp. 149–158, 2003. View at Publisher · View at Google Scholar · View at Scopus
  100. K. Šavikin, G. Zdunića, N. Menković et al., “Ethnobotanical study on traditional use of medicinal plants in South-Western Serbia, Zlatibor district,” Journal of Ethnopharmacology, vol. 146, no. 3, pp. 803–810, 2013. View at Publisher · View at Google Scholar
  101. L. Kokoska, Z. Polesny, V. Rada, A. Nepovim, and T. Vanek, “Screening of some Siberian medicinal plants for antimicrobial activity,” Journal of Ethnopharmacology, vol. 82, no. 1, pp. 51–53, 2002. View at Publisher · View at Google Scholar · View at Scopus
  102. F. Tetik, S. Civelek, and U. Cakilcioglu, “Traditional uses of some medicinal plants in Malatya (Turkey),” Journal of Ethnopharmacology, vol. 146, no. 1, pp. 331–346, 2013. View at Google Scholar
  103. W. Kisiel and K. Zielińska, “Guaianolides from Cichorium intybus and structure revision of Cichorium sesquiterpene lactones,” Phytochemistry, vol. 57, no. 4, pp. 523–527, 2001. View at Publisher · View at Google Scholar · View at Scopus
  104. J. S. Pyrek, “Sesquiterpene lactones of Cichorium intybus and Leontodon autumnalis,” Phytochemistry, vol. 24, no. 1, pp. 186–188, 1985. View at Google Scholar · View at Scopus
  105. P. Bridle, R. S. Thomas Loeffler, C. F. Timberlake, and R. Self, “Cyanidin 3-malonylglucoside in Cichorium intybus,” Phytochemistry, vol. 23, no. 12, pp. 2968–2969, 1984. View at Google Scholar · View at Scopus
  106. J. Malarz, A. Stojakowskaa, E. Sznelerb, and W. Kisiel, “A new neolignan glucoside from hairy roots of Cichorium intybus,” Phytochemistry Letters, vol. 6, pp. 59–61, 2013. View at Publisher · View at Google Scholar