Matrine Inhibits Infiltration of the Inflammatory Monocyte Subset in Injured Mouse Liver through Inhibition of Monocyte Chemoattractant Protein-1
Figure 7
Mat exhibited antifibrotic effects and inhibited hepatic infiltration of monocytes and production of MCP-1. C57BL/6 mice were injected i.p. with CCl4 (0.6 mL/kg, diluted in corn oil) or the same volume of corn oil twice weekly for 6 weeks. Mat (10 mg/kg) was orally administrated 5 times weekly for the last 3 weeks. Mice were sacrificed for analysis at 48 h after the last CCl4 injection. (a) Liver sections were stained with H&E, Sirius red, and Masson trichrome, respectively (original magnification ×100). (b) Liver hydroxyproline content was measured. (c) NPCs were isolated from livers, and the total number of NPCs, the percentage of CD45+ leukocytes in NPCs, the absolute number of CD45+ leukocytes, the percentage of different monocyte subsets in NPCs, and the absolute number of different monocyte subsets were analyzed with flow cytometry using the strategy as depicted in Figure 2. (d) Hepatic MCP-1 mRNA levels were analyzed with real-time PCR. Data in (b)–(d) are expressed as the mean ± (SD) ( mice/group). versus CCl4-only group; versus normal control group.
