Evidence-Based Complementary and Alternative Medicine / 2013 / Article / Fig 1

Research Article

Cytotoxic Activity of Six Samples of Brazilian Propolis on Sea Urchin (Lytechinus variegatus) Eggs

Figure 1

Micrographs evidencing the effect of methanol extract and fractions of samples of Brazilian propolis at 32 μg mL−1 on the initial development of sea urchin (Lytechinus variegatus) embryos. (a) control (ethanol): normal development; (b) effect of methanol extract of sample D: inhibition of egg cleavage; (c) effect of chloroform fraction of sample D: eggs with abnormal division; (d) effect of ethyl acetate fraction of sample A: eggs with abnormal division or inhibition of cleavage. A and D: samples from Minas Gerais state. For detailed information regarding the activity of extracts of all samples and extracts, see Table 1. Scale bars = 100 μm.

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