Evidence-Based Complementary and Alternative Medicine

Evidence-Based Complementary and Alternative Medicine / 2013 / Article

Research Article | Open Access

Volume 2013 |Article ID 636597 | https://doi.org/10.1155/2013/636597

Bo-Bae Kim, Jong Rhan Kim, Ji Hye Kim, Young Ah Kim, Jun Seong Park, Myeong-Hun Yeom, Hyong Joo Lee, Ki Won Lee, Nam Joo Kang, "7,3′,4′-Trihydroxyisoflavone Ameliorates the Development of Dermatophagoides farinae-Induced Atopic Dermatitis in NC/Nga Mice", Evidence-Based Complementary and Alternative Medicine, vol. 2013, Article ID 636597, 10 pages, 2013. https://doi.org/10.1155/2013/636597

7,3′,4′-Trihydroxyisoflavone Ameliorates the Development of Dermatophagoides farinae-Induced Atopic Dermatitis in NC/Nga Mice

Academic Editor: Xiu-Min Li
Received24 Jun 2013
Accepted07 Oct 2013
Published13 Nov 2013


Atopic dermatitis is an inflammatory and chronically relapsing skin disorder that commonly occurs in children; the number of atopic dermatitis patients is increasing. The cause and mechanism of atopic dermatitis have not been defined clearly, although many studies are ongoing. Epidemiological studies suggest that soybean and its isoflavones have immunoregulatory activities. Here, we report that 7,3′,4′-trihydroxyisoflavone (7,3′,4′-THIF), a major metabolite of daidzin, effectively inhibited lipopolysaccharide (LPS)-induced nitric oxide (NO), tumor necrosis factor (TNF)-α, and interleukin (IL)-6 production in RAW 264.7 cells, and also reduced β-hexosaminidase secretion in RBL-2H3 cells. Moreover, 7,3′,4′-THIF significantly reduced scratching time, transepidermal water loss, and mast cell infiltration. It also decreased protease-activated receptor (PAR)-2 and IL-4 expression and increased filaggrin expression in skin lesions of NC/Nga mice. These results suggest that 7,3′,4′-THIF improves Dermatophagoides farina body extract-induced atopic dermatitis in NC/Nga mice.

1. Introduction

Atopic dermatitis (AD) is a chronically relapsing skin disorder that presents with severe itching and inflammation [13]. It commonly occurs in children and infants, and its incidence is increasing globally [3, 4]. The cause and mechanism of AD are complex and have not been researched in detail; it is thought that AD is due to various factors, including genetic, immunologic, and environmental factors and dysfunction of the skin barrier [2].

Levels of various inflammatory mediators, including tumor necrosis factor (TNF)-α, nitric oxide (NO), and IL-6, are high in the skin of patients with AD [5]. TNF-α, NO, and IL-6 play important roles in inflammatory responses [5]. When allergens invade an AD skin lesion, invading allergen binds to specific IgE on the surface of mast cells or basophils and activates them to secrete substances such as histamine that are associated with allergic reactions [6]. Additionally, β-hexosaminidase is secreted from mast cells together with histamine and can be a marker of mast cell activation [7].

The skin barrier dysfunction is one of the major characteristics in AD patients [8]. PAR-2 is known to be involved in epidermal permeability barrier function homeostasis [9], and its activation was mediated by protease in mite allergens which can contribute to delay of epidermal barrier recovery [10]. Therefore, increased PAR-2 activity or expression was suggested as a pathophysiological factor for AD [11]. Filaggrin is a filament aggregating protein that binds to keratin fibers in epithelial cells and its degradation products such as free amino acids, PCA, and UCA are essential for skin moisture [12]. The loss-of-function variants of filaggrin are other major risk factors for AD [1315]. There are significant increases in the expression of IL-4, IL-5, and IL-13 mRNA and protein in acute AD lesions, suggesting the accumulation of Th2 cells [16]. IL-4, one of Th2 cytokines, plays an important role in the development of Th2 cells [17, 18], and previous studies indicated that IL-4 is a central mediator of allergic inflammation [19, 20].

Epidemiological studies suggest that soy isoflavones, popular components of soybeans, enhanced immunity [2124]. Daidzin and genistin are the major isoflavones found in soybeans [25]. The isoflavone glucoside (daidzin and genistin) must be hydrolyzed to aglycones (daidzein and genistein) by intestinal microorganisms to be absorbed [26]. Some previous studies suggest that genistein, a metabolite of genistin, inhibits the development of AD in NC/Nga mice [27]. Daidzin and its metabolites also show numerous benefits [28, 29]; however, there have been no reports of their possible anti-AD effects. Daidzein, a metabolite of daidzin, is further metabolized to two major components, 6,7,4′-trihydroxyisoflavone (6,7,4′-THIF) and 7,3′,4′-THIF, by the enzyme cytochrome P450 1A2 (CYP1A2) [30, 31]. Here, we investigated the antiatopic dermatitis effects of daidzin and 7,3′,4′-THIF in vitro or in vivo system.

2. Materials and Methods

2.1. Chemicals and Reagents

7,3′,4′-THIF was purchased from Indofine Chemical Co., Inc. (Hillsborough, NJ, USA). Daidzin and 3,3′-diaminobenzidine tetrahydrochloride hydrate were obtained from Sigma Chemical Co. (St. Louis, MO, USA). Enzyme-linked immunosorbent assay (ELISA) kits for mouse IL-6 and mouse TNF-α were obtained from BD Biosciences (San Jose, CA, USA). Anti-mouse antibodies against filaggrin and PAR-2 for immunohistochemistry were purchased from Santa Cruz Biotechnology, Inc. (Dallas, Texas, USA); an antibody against IL-4 was purchased from ProSpec (EB, NJ, USA). Blocking goat serum, a secondary antibody, and an ABC kit were obtained from Vector (Burlingame, CA, USA). Dermatophagoides farina body extract (DFE)-AD cream was purchased from Biostir Inc. (Hiroshima, Japan).

2.2. Cell Culture

Mouse RAW 264.7 macrophages and rat RBL-2H3 basophilic leukemia cells were obtained from the Korean Cell Line Bank (Seoul, Republic of Korea). Dulbecco’s modified Eagle’s medium (DMEM), penicillin-streptomycin, and 0.5% trypsin-EDTA were obtained from GIBCO Invitrogen (Auckland, New Zeland). Fetal bovine serum (FBS) was purchased from Sigma (St. Louis, MO, USA). RAW 264.7 cells and RBL-2H3 cells were cultured in DMEM supplemented with 10% FBS and 1% penicillin/streptomycin at 37°C in a humidified atmosphere containing 5% CO2.

2.3. Measurement of NO, TNF-α, IL-4, and IL-6 Levels In Vitro

RAW 264.7 cells were seeded at a density of 5 × 105 per well in a 96-well plate for NO assays and sandwich ELISA. After incubation for 24 h, the cells were stimulated with 2 μg/mL LPS in the presence or absence of 7,3′,4′-THIF or daidzin for the indicated period of time. Griess reagent was used to assay NO in culture supernatants. Briefly, 100 μL/well of sample was incubated at room temperature for 10 min with Griess reagent, which is a 1 : 1 mixture of 0.1% (w/v) N-1-naphthylethylenediamine and 1% (w/v) sulfanilamide in 5% phosphoric acid. The absorbance at 570 nm was measured. TNF-α, IL-4, and IL-6 levels were measured using ELISA kits according to the manufacturer’s instructions.

2.4. Measurement of β-Hexosaminidase Secretion In Vitro

RBL-2H3 cells were seeded at a density of 2 × 105 per well in a 24-well plate for β-hexosaminidase assays. After incubation for 24 h, the cells were treated with 0.5 μg/mL anti-DNP IgE solution (diluted in DMEM) for 2 h and then washed twice with 0.5 mL MT buffer (pH 7.3).

MT buffer (500 μL) was added in the presence or absence of 7,3′,4′-THIF or daidzin, and the cells were then incubated for 30 min at 37°C. Next, the cells were stimulated with DNP-BSA (final concentration 100 ng/mL) for 1 h and placed on ice, and the supernatants were transferred from the plate to microsample tubes. Aliquots of supernatants (50 μL) were transferred to 96-well plates for ELISA analysis. A 100-μL aliquot of the substrate solution was added to each supernatant well. The contents of the wells were mixed vigorously, and the plates were incubated at 37°C for 30 min. The enzymatic reaction was stopped by adding 100 μL of 2 M glycine buffer. The absorbance at 405 nm was evaluated according to the manufacturer’s instructions.

2.5. AD Induction and Administration of 7,3′,4′-THIF

Six-week-old male NC/Nga mice were purchased from Japan SLC, Inc. (Shizuoka, Japan). The mice were housed under SPF conditions (Center for Laboratory Animal Resources, Kyungpook National University) at a temperature of 22 ± 2°C and with a 12 h/12 h light-dark cycle during the entire experiment. After 3 weeks, the mice were randomly divided into four groups ( per group): an untreated control group, a DFE-treated group, and groups treated with DFE plus a low or high dose of 7,3′,4′-THIF (4 or 20 mg/kg). DFE-AD cream was applied twice per week for 10 weeks (50 mg per mouse per application). To disrupt the skin barrier, 200 μL of 4% (w/v) sodium dodecyl sulfate were topically applied to the shaved dorsal skin surface 3 h before DFE-AD cream application. All mice received 7,3′,4′-THIF or vehicle (2% DMSO/PBS) orally five times per week for 6 weeks.

All experiments with mice were performed in accordance with the regulations and approval of the Institutional Animal Care and Use Committee at Kyungpook National University.

2.6. Evaluation of Clinical Symptoms

Scratching time was observed for 20 min per mouse to evaluate itching severity. Ear thickness was measured with vernier calipers. Transepidermal water loss (TEWL) was measured using a Tewameter TM300 device (Courage + Khazaka Electronic GmbH, Germany) according to the manufacturer’s instructions.

2.7. Histopathological Examination

Dorsal skin lesions from each mouse were resected and fixed in 10% neutral-buffered formalin. Paraffin-embedded skin samples were sectioned to a thickness of 4 μm and stained with hematoxylin and eosin for assessment of epidermal thickness. Toluidine blue staining was performed to determine the number of mast cells. All analyses were performed under a microscope at 100x and 400x magnification.

2.8. Immunohistochemical Staining

Sections of formalin-fixed, paraffin-embedded tissue (4 μm thick) were cut, mounted on glass slides, deparaffinized, and rehydrated. Antigen retrieval was performed by incubation with trypsin working solution. To eliminate endogenous peroxidases, slides were incubated in 0.3% hydrogen peroxide in methanol. They were next blocked by incubation with 5% normal goat serum for 30 min, and then incubated with primary antibodies against PAR-2, filaggrin, and IL-4 at 4°C overnight. The sections were then developed using ABC solution. The reaction was visualized with 3,3′-diaminobenzidine tetrahydrochloride hydrate solution and the sections were finally counterstained with hematoxylin.

2.9. Statistics

Data are expressed as means ± standard deviation (SD) or standard error of the mean (SEM), and significant differences were identified using Student’s -test. values of <0.05, <0.01, and <0.001 were used as criteria for statistical significance.

3. Results

3.1. 7,3′,4′-THIF Inhibited LPS-Induced Cytokine Production in RAW 264.7 Cells and Anti-DNP IgE-Induced β-Hexosaminidase Production in RBL-2H3 Cells

Because various inflammatory cytokines are known to mediate inflammation and are highly expressed in AD patients [5], we first assessed the effects of 7,3′,4′-THIF (Figure 1(b)) and daidzin (Figure 1(a)) on LPS-induced NO, TNF-α, and IL-6 production in RAW 264.7 cells. 7,3′,4′-THIF dose-dependently inhibited the production of NO, whereas the same concentrations of daidzin had little effect (Figure 2(a)). Moreover, treatment with 7,3′,4′-THIF (40 μM), but not daidzin, inhibited LPS-induced TNF-α production (by 41.5%) (Figure 2(b)). Similar to above data, 40 μM 7,3′,4′-THIF reduced LPS-induced IL-6 production by 36.4%; 40 μM daidzin caused 10.7% inhibition (Figure 2(c)). Previous studies suggested that the secretion of β-hexosaminidase by mast cells is a marker of mast cell activation, which is associated with allergic reactions [6, 7]. Therefore, we next investigated the release of β-hexosaminidase from RBL-2H3 cells. The results show that 7,3′,4′-THIF significantly and dose-dependently reduced the secretion of β-hexosaminidase, whereas daidzin only slightly reduced β-hexosaminidase secretion (Figure 2(d)). These results suggest that 7,3′,4′-THIF has more potential than daidzin to suppress skin inflammation and mast cell activation.

3.2. 7,3′,4′-THIF Reduced the Clinical Symptoms in NC/Nga Mice

To investigate the clinical effect of 7,3′,4′-THIF on AD in NC/Nga mice, we assessed scratching time, ear thickness, and TEWL on day 70. The dorsal skin of the DFE-treated group showed AD symptoms such as erythema/hemorrhage, edema, excoriation/erosion, and scaling/dryness. The 7,3′,4′-THIF-treated groups showed dose-dependent improvement in visual features, and the high-dose-treated group showed a status similar to that of the untreated control group (Figure 3(b)). The reduction in dermatitis severity was accompanied by a reduced scratching incidence. 7,3′,4′-THIF administration greatly lowered the scratching time (31.5 ± 0.50 and 15 ± 2.00 sec) compared to the DFE-treated group (65 ± 1.00 sec) (Figure 3(c)). In addition, ear thickness was increased by DFE compared to the untreated control group. 7,3′,4′-THIF inhibited the DFE-induced increase in ear thickness, reducing ear thicknesses to levels similar to those in the untreated control group (Figure 3(d)). Moreover, these outcomes were consistent with those of TEWL. 7,3′,4′-THIF administration made the skin markedly moister compared to the DFE-treated group (Figure 3(e)). Treatment with low and high doses of 7,3′,4′-THIF caused 50.6 and 56.8% inhibition of DFE-induced TEWL, respectively. Overall, our results revealed that oral administration of 7,3′,4′-THIF suppressed the development of AD in NC/Nga mice.

3.3. 7,3′,4′-THIF Administration Reduced Epidermal Thickening and Mast Cell Infiltration in Skin Lesions

According to a previous study, the skin lesions of AD patients show characteristic features, including epidermal thickening and deepening into the upper dermis, in which mast cells were highly accumulated [32]. On day 77, all mice were killed and their dorsal skin was resected. The improvement of clinical features by 7,3′,4′-THIF was also confirmed by analysis of hematoxylin and eosin staining and toluidine blue staining. The DFE-treated group (37.3 ± 3.95 mm) exhibited an almost 2.5-fold increase in epidermal thickness compared to the untreated control group (16.1 ± 0.39 mm) (Figures 4(a) and 4(c)). A low or high dose of 7,3′,4′-THIF reduced the epidermis thickness to a level comparable to that in the untreated control group (17.7 ± 0.50 mm and 16.7 ± 0.83 mm). The infiltration of mast cells was also increased in the DFE-treated group (9.7 ± 1.10 cells per site), and 7,3′,4′-THIF decreased the number of mast cells (5.3 ± 0.46 and 4.9 ± 0.24 cells per site) to a level similar to that in the untreated control group (4.9 ± 0.25 cells per site) (Figures 4(b) and 4(d)). These results indicate that oral administration of 7,3′,4′-THIF suppressed DFE-induced AD-like skin lesions in NC/Nga mice by reducing epidermal thickness and the infiltration of mast cells.

3.4. 7,3′,4′-THIF Regulated PAR-2, Filaggrin, and Th2 Cytokine (IL-4) Expression in Skin Lesions in NC/Nga Mice

Recent studies revealed that aberrant expression and activation of PAR-2 and filaggrin mutations play crucial roles in the abnormal skin barrier function and skin lesions in AD patients [13]. Moreover, the skin barrier defect in AD is aggravated by atopic immune responses, including immune deviation versus Th2 dominance and increased IgE production [33]. The Th2 cytokine IL-4 is known to contribute to the development of Th2 cells and is overexpressed in AD-affected skin [1618]. Therefore, we performed immunohistochemical analysis of PAR-2, filaggrin, and IL-4 expression to determine the effects of 7,3′,4′-THIF. As shown in Figures 5(a) and 5(c), PAR-2 and IL-4 were highly expressed in the dermis in AD skin lesions in the DFE-treated group. Interestingly, 7,3′,4′-THIF significantly decreased the levels of PAR-2 and IL-4. Filaggrin expression was reduced in the DFE-treated group, while 7,3′,4′-THIF administration restored the expression of filaggrin (Figure 5(b)). Thus, by modulating PAR-2, filaggrin, and IL-4 expressions, 7,3′,4′-THIF might serve as a highly effective agent for improving AD-like skin lesions.

4. Discussion

Isoflavones are most abundant in soybeans, and are present in appreciable concentrations in various other beans, legumes, sprouts, clover, and alfalfa [28, 34]. In plants, isoflavones are mostly present as glucosides (daidzin and genistin). On ingestion, aglycones (daidzein and genistein) are liberated from glucosides following metabolism by intestinal microorganisms [34, 35]. These aglycones are absorbed more rapidly and in higher amounts than the corresponding glucosides in humans [36]. Several studies have shown that soybean extract and isoflavones have antiallergic effects [21, 22, 24]. The major aglycones daidzein and genistein were investigated to determine the impact of the Th2-polarized responses on allergic rhinitis in 1,002 pregnant women. The results suggest that consuming soy products abundant in isoflavones (tofu and cooked soybeans) significantly reduced symptoms of allergic rhinitis [23]. 7,3′,4′-THIF is a major metabolite of daidzein that is produced by hydroxylation of its 3′ carbon. Genistein was reported to have an anti-AD effect in NC/Nga mice [27], but daidzein and 7,3′,4′-THIF have not been investigated in a closed AD model. According to our results, 7,3′,4′-THIF had stronger inhibitory effects on the production of inflammatory cytokines and β-hexosaminidase. Based on this result, we suggest that 7,3′,4′-THIF inhibits skin inflammation and mast cell activation.

In this study, we demonstrated the effects of 7,3′,4′-THIF on AD using a DFE-induced AD model. 7,3′,4′-THIF improved visual clinical features, and reduced ear thickening and scratching behavior. It also inhibited TEWL. Furthermore, orally administered 7,3′,4′-THIF significantly inhibited epidermal thickening and infiltration of mast cells. The improved statuses were similar to those in the untreated control group.

Invasion of external allergens is an important risk factor for the development of AD. Allergens can invade the skin more easily if the skin barrier is disrupted. As previously mentioned, PAR-2 and filaggrin have a crucial function in maintaining epidermal barrier homeostasis [37]. Levels of the endogenous PAR-2 agonist tryptase were increased up to fourfold in skin lesions of AD patients [10]. Topical application of PAR-2 activator peptides to affected skin was reported to delay barrier recovery [11]. PAR-2 is also related to itch, and its levels on primary afferent nerve fibers in skin biopsies from AD patients were markedly increased [10]. PAR-2 is expressed during immunologic reactions and it helps dendritic cells (DCs) to mature [13, 38]. Intracutaneous injection of PAR-2 agonists provoked increased and prolonged itch, and the PAR-2-mediated itch pathway provides a link to therapies for pruritus [10].

Filaggrin is a filament aggregating protein that binds to keratin fibers in epithelial cells. It aggregates the keratin filaments into tight bundles and its degradation products such as free amino acids, PCA, and UCA are essential for skin moisture [13]. According to our results, 7,3′,4′-THIF suppressed PAR-2 expression and increased the expression of filaggrin. 7,3′,4′-THIF relieves itch and maintains skin barrier moisture. AD is provoked by an imbalance between the Th1 and Th2 immune responses. Th2 cell-related responses are mediated by the Th2 cytokines IL-4, IL-5, and IL-13, whereas Th1 cell-related responses are mediated by IFN-γ [4, 39]. Notably, Th2 cytokines are key factors in the progression of inflammatory and immunologic skin disorders to chronic diseases such as AD. These cytokines have a strong association with the onset and aggravation of AD. Indeed, cytokine levels are increased in AD-like skin lesions. Therefore, Th2 normalization is important for relief of AD symptoms. 7,3′,4′-THIF also regulated the expression of one of Th2 cytokines, IL-4.

In patients with AD, serum IgE levels are well correlated with disease severity [40]. In this study, IgE levels in the AD group were significantly increased compared with those in the noninduction group; however, 7,3′,4′-THIF had no effect on serum IgE level (data not shown).

5. Conclusions

We demonstrated that 7,3′,4′-THIF improved AD symptoms in DFE-treated NC/Nga mice. We expect our findings to be applicable to the improvement of AD. Consumption of soybeans, which have long been a part of the diet in Asian countries, and their ingredients have recently increased globally. Development and consuming of 7,3′,4′-THIF-rich soybeans would facilitate prevention or management of AD and other Th2-polarized diseases according to the selective needs of the individual.


AD: Atopic dermatitis
6,7,4′-THIF: 6,7,4′-trihydroxyisoflavone
7,3′,4′-THIF: 7,3′,4′-trihydroxyisoflavone
DFE:Dermatophagoides farinae body extract
IL: Interleukin
LPS: Lipopolysaccharides
NO: Nitric oxide
PAR-2: Protease activated receptor-2
SPF: Specific pathogen free
TNF-α: Tumor necrosis factor-α.

Conflict of Interests

All authors declare that they have no conflict of interests.

Authors’ Contribution

Bo-Bae Kim and Jong Rhan Kim contributed equally to this work.


This research was supported by the Next-Generation BioGreen 21 Program (PJ008060, PJ009542), the Rural Development Administration; by the National Research Foundation of Korea (NRF) grant (2010-0027204), the Korea government; by 2012 High Value Added Food Industry Professional Human Resources Development Project, Ministry of Agriculture, Food, and Rural Affairs, Republic of Korea.


  1. S. Dold, M. Wjst, E. von Mutius, P. Reitmeir, and E. Stiepel, “Genetic risk for asthma, allergic rhinits, and atopic dermatitis,” Archives of Disease in Childhood, vol. 67, no. 8, pp. 1018–1022, 1992. View at: Google Scholar
  2. H. Suto, H. Matsuda, K. Mitsuishi et al., “NC/Nga mice: a mouse model for atopic dermatitis,” International Archives of Allergy and Immunology, vol. 120, no. 1, pp. 70–75, 1999. View at: Publisher Site | Google Scholar
  3. G. Wu, L. Li, G. H. Sung et al., “Inhibition of 2,4-dinitrofluorobenzene-induced atopic dermatitis by topical application of the butanol extract of Cordyceps bassiana in NC/Nga mice,” Journal of Ethnopharmacology, vol. 134, no. 2, pp. 504–509, 2011. View at: Publisher Site | Google Scholar
  4. T. Oshio, Y. Sasaki, M. Funakoshi-Tago et al., “Dermatophagoides farinae extract induces severe atopic dermatitis in NC/Nga mice, which is effectively suppressed by the administration of tacrolimus ointment,” International Immunopharmacology, vol. 9, no. 4, pp. 403–411, 2009. View at: Publisher Site | Google Scholar
  5. Y. Mutou, Y. Ibuki, and S. Kojima, “Immunomodulatory effects of ultraviolet B irradiation on atopic dermatitis in NC/Nga mice,” Photodermatology Photoimmunology and Photomedicine, vol. 23, no. 4, pp. 135–144, 2007. View at: Publisher Site | Google Scholar
  6. E. V. Ponte, J. A. Rizzo, and A. A. Cruz, “Interrelationship among asthma, atopy, and helminth infections,” Jornal Brasileiro de Pneumologia, vol. 33, no. 3, pp. 335–342, 2007. View at: Publisher Site | Google Scholar
  7. H. Mastuda, T. Morikawa, K. Ueda, H. Managi, and M. Yoshikawa, “Structural requirements of flavonoids for inhibition of antigen-Induced degranulation, TNF-α and IL-4 production from RBL-2H3 cells,” Bioorganic and Medicinal Chemistry, vol. 10, no. 10, pp. 3123–3128, 2002. View at: Publisher Site | Google Scholar
  8. M. J. Cork, D. A. Robinson, Y. Vasilopoulos et al., “New perspectives on epidermal barrier dysfunction in atopic dermatitis: gene-environment interactions,” Journal of Allergy and Clinical Immunology, vol. 118, no. 1, pp. 3–21, 2006. View at: Publisher Site | Google Scholar
  9. S. K. Jeong, H. J. Kim, J. K. Youm et al., “Mite and cockroach allergens activate protease-activated receptor 2 and delay epidermal permeability barrier recovery,” Journal of Investigative Dermatology, vol. 128, no. 8, pp. 1930–1939, 2008. View at: Publisher Site | Google Scholar
  10. M. Steinhoff, U. Neisius, A. Ikoma et al., “Proteinase-activated receptor-2 mediates itch: a novel pathway for pruritus in human skin,” Journal of Neuroscience, vol. 23, no. 15, pp. 6176–6180, 2003. View at: Google Scholar
  11. J. P. Hachem, E. Houben, D. Crumrine et al., “Serine protease signaling of epidermal permeability barrier homeostasis,” Journal of Investigative Dermatology, vol. 126, no. 9, pp. 2074–2086, 2006. View at: Publisher Site | Google Scholar
  12. J. A. McGrath and J. Uitto, “The filaggrin story: novel insights into skin-barrier function and disease,” Trends in Molecular Medicine, vol. 14, no. 1, pp. 20–27, 2008. View at: Publisher Site | Google Scholar
  13. B. E. Kim and D. Y. M. Leung, “Epidermal barrier in atopic dermatitis,” Allergy, Asthma and Immunology Research, vol. 4, no. 1, pp. 12–16, 2012. View at: Publisher Site | Google Scholar
  14. C. N. A. Palmer, A. D. Irvine, A. Terron-Kwiatkowski et al., “Common loss-of-function variants of the epidermal barrier protein filaggrin are a major predisposing factor for atopic dermatitis,” Nature Genetics, vol. 38, no. 4, pp. 441–446, 2006. View at: Publisher Site | Google Scholar
  15. G. M. O'Regan, A. Sandilands, W. H. I. McLean, and A. D. Irvine, “Filaggrin in atopic dermatitis,” Journal of Allergy and Clinical Immunology, vol. 124, no. 3, pp. R2–R6, 2009. View at: Publisher Site | Google Scholar
  16. J. M. Spergel, E. Mizoguchi, H. Oettgen, A. K. Bhan, and R. S. Geha, “Roles of T(H)1 and T(H)2 cytokines in a murine model of allergic dermatitis,” Journal of Clinical Investigation, vol. 103, no. 8, pp. 1103–1111, 1999. View at: Google Scholar
  17. U. Herz, R. Bunikowski, and H. Renz, “Role of T cells in atopic dermatitis. New aspects on the dynamics of cytokine production and the contribution of bacterial superantigens,” International Archives of Allergy and Immunology, vol. 115, no. 3, pp. 179–190, 1998. View at: Publisher Site | Google Scholar
  18. D. Vercelli and R. S. Geha, “Regulation of isotype switching,” Current Opinion in Immunology, vol. 4, no. 6, pp. 794–797, 1992. View at: Publisher Site | Google Scholar
  19. R. I. Tepper, D. A. Levinson, B. Z. Stanger, J. Campos-Torres, A. K. Abbas, and P. Leder, “IL-4 induces allergic-like inflammatory disease and alters T cell development in transgenic mice,” Cell, vol. 62, no. 3, pp. 457–467, 1990. View at: Publisher Site | Google Scholar
  20. G. G. Brusselle, J. C. Kips, J. H. Tavernier et al., “Attenuation of allergic airway inflammation in IL-4 deficient mice,” Clinical and Experimental Allergy, vol. 24, no. 1, pp. 73–80, 1994. View at: Publisher Site | Google Scholar
  21. K. Pałgan and Z. Bartuzi, “The role of flavonoids in asthma,” Postępy Dermatologii i Alergologii, vol. 28, pp. 396–401, 2011. View at: Google Scholar
  22. K. Yasui, H. Ikemoto, K. Taguchi et al., “The clinical and immunomodulatory effects of green soybean extracts,” Food Chemistry, vol. 138, no. 4, pp. 2300–2305, 2013. View at: Publisher Site | Google Scholar
  23. Y. Miyake, S. Sasaki, Y. Ohya et al., “Soy, isoflavones, and prevalence of allergic rhinitis in Japanese women: the Osaka maternal and child health study,” Journal of Allergy and Clinical Immunology, vol. 115, no. 6, pp. 1176–1183, 2005. View at: Publisher Site | Google Scholar
  24. T. Zhang, W. Pan, M. Takebe, B. Schofield, H. Sampson, and X. M. Li, “Therapeutic effects of a fermented soy product on peanut hypersensitivity is associated with modulation of T-helper type 1 and T-helper type 2 responses,” Clinical and Experimental Allergy, vol. 38, no. 11, pp. 1808–1818, 2008. View at: Publisher Site | Google Scholar
  25. A. C. Eldridge and W. F. Kwolek, “Soybean isoflavones: effect of environment and variety on composition,” Journal of Agricultural and Food Chemistry, vol. 31, no. 2, pp. 394–396, 1983. View at: Google Scholar
  26. J. Chun, G. M. Kim, K. W. Lee et al., “Conversion of isoflavone glucosides to aglycones in soymilk by fermentation with lactic acid bacteria,” Journal of Food Science, vol. 72, no. 2, pp. M39–M44, 2007. View at: Publisher Site | Google Scholar
  27. T. Sakai, M. Kogiso, K. Mitsuya, T. Komatsu, and S. Yamamoto, “Genistein suppresses development of spontaneous atopic-like dermatitis in NC/Nga mice,” Journal of Nutritional Science and Vitaminology, vol. 52, no. 4, pp. 293–296, 2006. View at: Google Scholar
  28. D. E. Lee, K. W. Lee, S. Byun et al., “7,3′,4′-trihydroxyisoflavone, a metabolite of the soy isoflavone Daidzein, suppresses ultraviolet B-induced skin cancer by targeting Cot and MKK4,” Journal of Biological Chemistry, vol. 286, no. 16, pp. 14246–14256, 2011. View at: Publisher Site | Google Scholar
  29. Y. L. Lo, W. Wang, and C. T. Ho, “7,3′,4′-trihydroxyisoflavone modulates multidrug resistance transporters and induces apoptosis via production of reactive oxygen species,” Toxicology, vol. 302, no. 2-3, pp. 221–232, 2012. View at: Publisher Site | Google Scholar
  30. S. E. Kulling, D. M. Honig, and M. Metzler, “Oxidative metabolism of the soy isoflavones daidzein and genistein in humans in vitro and in vivo,” Journal of Agricultural and Food Chemistry, vol. 49, no. 6, pp. 3024–3033, 2001. View at: Google Scholar
  31. K. M. Atherton, E. Mutch, and D. Ford, “Metabolism of the soyabean isoflavone daidzein by CYP1A2 and the extra-hepatic CYPs 1A1 and 1B1 affects biological activity,” Biochemical Pharmacology, vol. 72, no. 5, pp. 624–631, 2006. View at: Publisher Site | Google Scholar
  32. H. Matsuoka, N. Maki, S. Yoshida et al., “A mouse model of the atopic eczema/dermatitis syndrome by repeated application of a crude extract of house-dust mite Dermatophagoides farinae,” Allergy, vol. 58, no. 2, pp. 139–145, 2003. View at: Publisher Site | Google Scholar
  33. J. Ring, M. Mhrenschlager, and S. Weidinger, “Molecular genetics of atopic eczema,” Chemical Immunology and Allergy, vol. 96, pp. 24–29, 2012. View at: Publisher Site | Google Scholar
  34. S. E. Kulling, L. Lehmann, and M. Metzler, “Oxidative metabolism and genotoxic potential of major isoflavone phytoestrogens,” Journal of Chromatography B, vol. 777, no. 1-2, pp. 211–218, 2002. View at: Publisher Site | Google Scholar
  35. N. G. Coldham and M. J. Sauer, “Pharmacokinetics of [14C]genistein in the rat: gender-related differences, potential mechanisms of biological action, and implications for human health,” Toxicology and Applied Pharmacology, vol. 164, no. 2, pp. 206–215, 2000. View at: Publisher Site | Google Scholar
  36. T. Izumi, M. K. Piskula, S. Osawa et al., “Soy isoflavone aglycones are absorbed faster and in higher amounts than their glucosides in humans,” Journal of Nutrition, vol. 130, no. 7, pp. 1695–1699, 2000. View at: Google Scholar
  37. S. Weidinger, T. Illig, H. Baurecht et al., “Loss-of-function variations within the filaggrin gene predispose for atopic dermatitis with allergic sensitizations,” Journal of Allergy and Clinical Immunology, vol. 118, no. 1, pp. 214–219, 2006. View at: Publisher Site | Google Scholar
  38. C. Ebeling, T. Lam, J. R. Gordon, M. D. Hollenberg, and H. Vliagoftis, “Proteinase-activated receptor-2 promotes allergic sensitization to an inhaled antigen through a TNF-mediated pathway,” Journal of Immunology, vol. 179, no. 5, pp. 2910–2917, 2007. View at: Google Scholar
  39. D. Hijnen, E. Nijhuis, M. de Bruin-Weller et al., “Differential expression of genes involved in skin homing, proliferation, and apoptosis in CD4+ T cells of patients with atopic dermatitis,” Journal of Investigative Dermatology, vol. 125, no. 6, pp. 1149–1155, 2005. View at: Publisher Site | Google Scholar
  40. F. T. Liu, H. Goodarzi, and H. Y. Chen, “IgE, mast cells, and eosinophils in atopic dermatitis,” Clinical Reviews in Allergy and Immunology, vol. 41, no. 3, pp. 298–310, 2011. View at: Publisher Site | Google Scholar

Copyright © 2013 Bo-Bae Kim et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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