7,3′,4′-Trihydroxyisoflavone Ameliorates the Development of Dermatophagoides farinae-Induced Atopic Dermatitis in NC/Nga Mice

Figure 4

Histological features of AD skin lesions in NC/Nga mice. (a) Hematoxylin and eosin staining features of skin lesions. Epidermal hyperplasia was evaluated in randomized three sites under a microscope at 100x magnification. Scale bar: 50 μm. (b) Toluidine blue staining for assessment of mast cell infiltration in skin lesions. Mast cells were counted in three random 0.025 mm² sites under a microscope at 400x magnification. Scale bar: 50 μm. Data represent means ± SEM (𝑛=5). *𝑃<0.05, **𝑃<0.01, and ***𝑃<0.001 compared with DFE-treated mice. (A) Untreated control group; (B) DFE-treated group; (C) DFE plus low-dose 7,3′,4′-THIF (4 mg/kg); (D) DFE plus high-dose 7,3′,4′-THIF (20 mg/kg).

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