Freshwater Clam Extract Ameliorates Triglyceride and Cholesterol Metabolism through the Expression of Genes Involved in Hepatic Lipogenesis and Cholesterol Degradation in Rats
Figure 1
Hepatic lipids, index of hepatic elongation of palmitate and palmitoleate, and desaturation index of palmitate and stearate in rats fed FCE and SPI. In rats fed control diet, FCE, or SPI, hepatic lipids were extracted, and triglycerides, cholesterol, and phospholipids were quantified using commercial kits (a). Extracted hepatic lipids were converted to methyl ester, and the fatty acid profile was analyzed using gas chromatography/mass spectrometry. Then, fatty acid ratios were calculated to estimate the elongation of palmitate and palmitoleate (C18:0/C16:0, C18:1/C16:1) and desaturation of palmitate and stearate (C16:1/C16:0, C18:1/C18:0) (b). The statistical significance of differences among values was analyzed by ANOVA and then by Tukey’s multiple-range test. Values with different letters indicate a statistically significant difference, . Each value is expressed as the mean ± SEM for 6 rats in each group (control (C), FCE (F), SPI (S)).
