Administration Dependent Antioxidant Effect of Carica papaya Seeds Water Extract
Figure 5
Detroit 550 fibroblasts labelled with the fluorescent probe JC-1 after the treatment with the C. papaya seeds water extract and/or with H2O2. Fibroblasts were labelled with JC-1 (1% v/v) and green or red fluorescence was observed with an epifluorescence LM by setting a FITC (a, c, e, g, i, k, m, and o) or TRITC filter (b, d, f, h, j, l, n, and p). (a)–(d): control untreated fibroblasts at T0 (a, b) and at 4 h (c-d); (e)-(f): fibroblasts treated with 1 mg/mL of C. papaya (Cp) extract for 4 h; (g)-(h): fibroblasts incubated with H2O2 1 mM for 1 h; ((i)-(j)): fibroblasts incubated with 1 mM H2O2 and 1 mg/mL of Cp simultaneously for 1 h; (k)-(l): fibroblasts incubated with 1 mM H2O2 for 1 h and with 1 mg/mL of Cp extract added during recovery; (m)-(n): cell treated with 1 mg/mL of the subfraction with kDa (see Figure 1); (o)-(p): fibroblasts simultaneously incubated with 1 mg/mL of the subfraction with kDa and 1 mM H2O2 for 1 h. Bars = 20 μm.
