Hydrolysis of Glycosidic Flavonoids during the Preparation of Danggui Buxue Tang: An Outcome of Moderate Boiling of Chinese Herbal Mixture
Figure 4
A time-dependent hydrolysis of flavonoid glycosides in the present of herbal powder. Under 25°C, 50 μg of calycosin-7-O-β-D-glucoside (a), or 50 μg ononin (b), was included to the water (50 mL) suspended with powders (1 g) of ASR or AR. Every hour, 0.5 mL of the solution was taken out. The amounts of calycosin-7-O-β-D-glucoside, calycosin, ononin, formononetin, and astragaloside IV were determined by RRLC-QQQ-MS/MS as described in Figure 1. The percentage of hydrolysis rate was determined by the amount of flavonoid or flavonoid glycoside (in molar) determined to that original applied flavonoid glycoside (in molar). Control does not have herbal powder. The values are in Mean ± SD, where , each with triplicate samples.
