An In Vitro System Comprising Immortalized Hypothalamic Neuronal Cells (GT1–7 Cells) for Evaluation of the Neuroendocrine Effects of Essential Oils
Cytotoxicity of H2O2, Al, Zn, and TMX. GT1–7 cells were treated with 20–50 μM H2O2 (gray), 50–200 μM Al (stripes), 10–50 μM Zn (black), or 0.1–0.5 μM TMX (lattice). After 24 h, the viability was analyzed using the WST-1 method. The white bar shows the viability of GT1–7 cells treated with essential oil alone. Data are presented as the mean ± SEM (). , versus the control group. The abbreviated names of the essential oils used in the figure are shown in Table 1.