Synergism Effect of the Essential Oil from Ocimum basilicum var. Maria Bonita and Its Major Components with Fluconazole and Its Influence on Ergosterol Biosynthesis

Figure 6

Transmission electron microscopy of C. albicans fluconazole resistant strain treated with 625 μg/mL of EO and 76 μg/mL of geraniol for 48 h at 37°C. Control cells showed good preservation of the membrane, cell wall, and small vesicles near the membrane (a and b). Yeasts treated with subinhibitory concentrations of the essential oil and geraniol showed cell wall thickening and irregularities in the membrane (c and d). Cells treated with subinhibitory concentration of geraniol presented cell wall thickening and irregularities in cell membrane, suggesting budding failure (e and f).

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