Electroacupuncture at GV20 and ST36 Exerts Neuroprotective Effects via the EPO-Mediated JAK2/STAT3 Pathway in Cerebral Ischemic Rats

Figure 6

Double-labeling immunofluorescent localization of JAK2 and cellular markers (NeuN and GFAP) 24 h after CIRI in the M group is shown in Figure 6. The colocalization of TUNEL and EPO is also shown. Many neurons in the M group colocalized with JAK2 in Figures 6(D)–6(F) (red arrows), and JAK2 immunofluorescence was most often dissociated from GFAP immunofluorescence in Figures 6(A)–6(C) (white arrows). Purple arrows showed nearly complete dissociation of EPO and TUNEL-positive cells (Figures 6(G)–6(I)). Scale bar = 50 μm. The calculation of PCC for JAK2 and GFAP was less than 20%, while the value for JAK2 and NeuN was more than 80%. The observation of dissociation between TUNEL-positive cells and EPO-labeled cells was supported by the results of coefficients calculation: PPC was less than 10%.

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