Effects of the Fruit Extract of Tribulus terrestris on Skin Inflammation in Mice with Oxazolone-Induced Atopic Dermatitis through Regulation of Calcium Channels, Orai-1 and TRPV3, and Mast Cell Activation

Figure 5

Effects of TF extract on calcium channels in HEK293T cells. Orai-1 current () in HEK293T cells coexpressing Orai-1/STIM1 (a). Representative chart trace recordings of currents (A). The number of parentheses represents steady state (1) and its inhibition by 1 mg/mL of TF extract (2). Related I-V relationships of (1) and of (2) in (b). Summary of inhibition rate of by TF extract at −120 mV (C). The results are expressed as the mean ± SD (). versus . Effects of TF extract on activation of TRPV3 in HEK293T cells overexpressing TRPV3 (b). Representative chart recording of activation by TF extract (A). The number of parentheses represents steady state TRPV3 current () after TF extract treatment (1) and the maximal current induced by 50 μM 2-APB () (2). Related current-voltage (I-V) relationship curve at (1) and (2) from Figure 6(b). Normalized graph of activation by TF extract and 2-APB at −100 mV (C). The results are expressed as the mean ± SD (). versus .

(a)

(b)