Activation of AMPK by Buddleja officinalis Maxim. Flower Extract Contributes to Protecting Hepatocytes from Oxidative Stress

Figure 4

Effects of BFE on AMPK activation. (a) AMPK activation in cell lysate treated with 30 μg/mL of BFE for the indicated time periods. Equal protein loading was verified by β-actin immunoblotting. A representative blot from three independent experiments is shown. Relative protein levels of activated AMPK were analyzed by densitometry. (b) The role of AMPK on BFE-mediated cytoprotection. Cell viability was performed on HepG2 cells incubated without or with 10 μM of compound C for 1 h and continuously treated with BFE (10 or 30 μg/mL, 6 h) and AA + iron, as described in Figure 2(a). Data represent means ± SD of three independent experiments [(a) between the control and BFE-treated cells; (b) between control and AA + iron-treated cells; and between AA + iron-treated cells without or with BFE or compound C].

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