Phosphatidylinositide 3-Kinase Contributes to the Anti-Inflammatory Effect of Abutilon crispum L. Medik Methanol Extract

Figure 1

Effect of Ac-ME on production of inflammatory mediators and HPLC profiling of Ac-ME. (a) Levels of NO production induced by Pam3CSK4 (a TLR2 ligand) and LPS (a TLR 4 ligand) were determined by the Griess Assay from supernatant of RAW264.7 cell treated with Ac-ME (left panel) or prednisolone (Pred) (right panel) for 24 h. (b) Cell viability of RAW264.7 and HEK293 cells treated with Ac-ME (left panel) or Pred (right panel) was determined by MTT assay. (c) The phytochemical profile of Ac-ME was analyzed by HPLC with the conditions described in Table 1 (left panel). The content of flavonoids was shown in the graph by calculating from the standard curves of the compounds (right panel). P < 0.05 and P < 0.01 compared to the control.

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