Src Is a Prime Target Inhibited by Celtis choseniana Methanol Extract in Its Anti-Inflammatory Action
Effects of Cc-ME on NO production and cytotoxicity in macrophages and HPLC analysis of Cc-ME components. (a) RAW264.7 cells (left panel) or peritoneal macrophages (right panel) pretreated with the indicated doses of Cc-ME or L-NAME were treated with LPS (1 μg/ml), pam3 (10 μg/ml), or poly(I:C) (200 μg/ml) for 24 h, and NO production level in the cells was determined by the Griess assay. (b) RAW264.7 cells or peritoneal macrophages were treated with the indicated doses of Cc-ME or L-NAME for 24 h, and the viabilities of the cells were determined by the MTT assay. (c) The NO inhibitory activity (left panel) of prednisolone (Pred) and its cytotoxicity (right panel) were examined by NO assay and MTT assay. (d) The phytochemical profile (left panel) of Cc-ME was analyzed by HPLC using standard compounds (quercetin, luteolin, and kaempferol) in the conditions described in Table 1, and the contents of the flavonoids (right panel) were calculated from the standard curves of the standard compounds. The data presented in (a), (b), and (c) are expressed as the means ± SD of experiments that were performed with six. and compared to normal group; and compared to control group.
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