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Evidence-Based Complementary and Alternative Medicine
Volume 2018, Article ID 4360356, 10 pages
Research Article

Hypoglycaemic and Antioxidant Effects of Propolis of Chihuahua in a Model of Experimental Diabetes

1Lab. Inmunobiología (L-321), UNAM FES Iztacala, Avenida de los Barrios Número 1, Colonia Los Reyes Iztacala, 54090 Tlalnepantla, MEX, Mexico
2IPN, Escuela Nacional de Ciencias Biológicas, Av. Wilfrido Massieu, Gustavo A Madero, 07738 Ciudad de México, Mexico
3Instituto de Química, Universidad Nacional Autónoma de México, Circuito Exterior, Ciudad Universitaria, 04510 Coyoacán, DF, Mexico
4UBIMED, UNAM FES Iztacala, Avenida de los Barrios Número 1, Colonia Los Reyes Iztacala, 54090 Tlalnepantla, MEX, Mexico
5Lab. Farmacognosia, UBIPRO, UNAM FES Iztacala, Carrera Biología, Tlalnepantla, MEX, Mexico

Correspondence should be addressed to M. A. Rodriguez-Monroy; moc.liamg@yornomzeugirdorocram.rd

Received 8 August 2017; Revised 21 December 2017; Accepted 7 February 2018; Published 11 March 2018

Academic Editor: Eman Al-Sayed

Copyright © 2018 Nelly Rivera-Yañez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Propolis is a bee-collected natural product that has been proven to have various bioactivities. This study tested the effects of a Mexican propolis on streptozotocin-induced diabetes mellitus in a murine model. The results showed that an ethanolic extract of propolis of Chihuahua (EEPCh) significantly inhibited increases in blood glucose and the loss of body weight in diabetic mice. EEPCh increased plasma insulin levels in STZ-diabetic mice, whereas, in untreated diabetic mice, there was no detection of insulin. EEPCh had a high antioxidant capacity (SA50 = 15.75 μg/mL), which was directly related to the concentrations of total phenols (314 mg GAE/g of extract) and flavonoids (6.25 mg QE/g of extract). In addition, increased activities of the enzymes superoxide dismutase, catalase, and glutathione peroxidase were observed in diabetic mice treated with EEPCh. Compounds such as pinocembrin, quercetin, naringin, naringenin, kaempferol, acacetin, luteolin, and chrysin were identified by HPLC-MS analysis. This investigation demonstrated that propolis of Chihuahua possesses hypoglycaemic and antioxidant activities and can alleviate symptoms of diabetes mellitus in mice. These effects may be directly related to the chemical composition of propolis, as most of the compounds identified in propolis are reportedly active in terms of the different parameters evaluated in this work.