The Antineuroinflammatory Effect of Simvastatin on Lipopolysaccharide Activated Microglial Cells
Table 1
Cytotoxic effect of LPS, simvastatin, and LPS+ simvastatin on rat C6 glioma cell lines at different concentrations and incubation times. Data is expressed as the mean ± SD (each treatment, n = 3). Nonsignificant. indicates p < 0.001 in comparison with control/untreated cells by one-way ANOVA followed by Dunnett’s test for multiple comparison.
Drug (Test compound)
Concentration
Cell viability ()
Incubation Time
24 h
48 h
Camptothecin (Positive control)
15 µM
50.45 ± 0.09
27.14 ± 0.10
Control/untreated cell line (Negative control)
-
100 ± 0.005
100 ± 0.005
LPS
3.125 µg/mL
99.89 ± 0.12
95.20 ± 0.09
6.25 µg/mL
96.90 ± 0.11
89.10 ± 0.07
12.5 µg/mL
94.60 ± 0.08
85.20 ± 0.08
25 µg/mL
90.10 ± 0.10
77.50 ± 0.06
50 µg/mL
87.30 ± 0.06
72.80 ± 0.08
100 µg/mL
85.50 ± 0.09
69.50 ± 0.04
Simvastatin
6.25 µM
98.10 ± 0.10
85.80 ± 0.09
12.5 µM
96.30 ± 0.09
83.00 ± 0.08
25 µM
92.17 ± 0.07
79.30 ± 0.10
50 µM
90.07 ± 0.10
75.20 ± 0.09
100 µM
86.90 ± 0.09
70.80 ± 0.09
LPS (3.125 µg/mL) + Simvastatin at different concentrations
