Research Article

Scrodentoids H and I, a Pair of Natural Epimerides from Scrophularia dentata, Inhibit Inflammation through JNK-STAT3 Axis in THP-1 Cells

Figure 5

Scrodentoids H and I decreased the phosphorylation of JNK rather than ERK and P38. THP-1 cells were pretreated with various concentrations of SHI and SP600125 for 1 h following treatment of LPS (1 μg/mL) for 30 mins. (a, b) Total proteins were extracted for western blot analysis to determine the expression levels of p-ERK, ERK, p-P38, P38, p-JNK, and JNK. (c) It is a statistical result of three independent experiments. All data are mean ± SEM, n = 3, vs. LPS-stimulated cells and vs. control.