Dehydrocorydaline Accounts the Majority of Anti-Inflammatory Property of Corydalis Rhizoma in Cultured Macrophage

Figure 5

CR alkaloids inhibit transcription of proinflammatory cytokines. Cultured RAW 264.7 cells in 24-well plates were treated with different doses of dehydrocorydaline, corydaline, and corydalmine, as described in Figure 3. The cultured medium was collected and tested for protein levels of proinflammatory cytokines by ELISA, and the cells were harvested for mRNA expression of proinflammatory cytokines by real-time PCR. (a) The protein (left vertical axis) and mRNA (right vertical axis) levels of TNF-α. (b) The protein and mRNA levels of IL-6. (c) The mRNA level of IL-1β. Data are expressed as fold of change to basal (no drug), in mean ± SEM, n = 3, each with triplicate samples. and , compared to model control (LPS but no CR alkaloid).

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