Dehydrocorydaline Accounts the Majority of Anti-Inflammatory Property of Corydalis Rhizoma in Cultured Macrophage

Figure 7

CR alkaloids suppress LPS-induced translocation of NF-κB. Cultured RAW 264.7 cells were treated with dehydrocorydaline, corydaline, and corydalmine at concentrations of 20, 60, and 90 µM, respectively, as described in Figure 3. Immunofluorescence staining was applied to observe the localization of NF-κB p65 by antibody (red fluorescence) and in nuclei staining by DAPI (blue fluorescence) in RAW 264.7 cells. Asterisks indicated nuclei with NF-κB staining, and also the enlarged cell was in the bottom right corner.