Protective Effect of Simplicillium sp. Ethyl Acetate Extract against High Glucose-Induced Oxidative Stress in HUVECs

Figure 8

CH increased the Nrf2 protein level in HG-induced HUVECs. (a) Cells were pretreated with/without 80 nM bardoxolone for 1.5 h, followed by incubating with/without 32 μM CH for 24 h Western blot analysis of Nrf2 protein levels after treatment. (b) Cells were pretreated with/without 5 µM ML385 for 1.5 h, followed by incubating with/without 32 μM CH for 24 h. Western blot analysis of Nrf2 protein levels after treatment. (c) Using siRNA technology to silent Nrf2. After 24 hours of treatment, Nrf2 was knocked down by siRNA (siRNA-Nrf2), and then, these cells were exposed to normal medium for 24 h, respectively. A disordered sequence siRNA (siRNA-NC) was transfected into HUVECs, and then, these cells were exposed to normal medium for 24 h. Results were represented as the mean ± SEM (n = 3). vs. control group; vs. control group; vs. model group; vs. HG + bardoxolone or HG + ML385 group; ^$$ vs. HG + CH + NC group; vs. HG + siNrf2 group.

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