2-(6-Hydroxyhexylthio)-5,8-dimethoxy-1,4-naphthoquinone Induces Apoptosis through ROS-Mediated MAPK, STAT3, and NF-κB Signalling Pathways in Lung Cancer A549 Cells

Figure 2

Effects of HEDMNQ and HHDMNQ on cell growth in human lung cancer cells. (a) Cell viabilities of A549, NCI-H23, and NCI-H460 cells. (b) Cell viabilities of normal lung IMR-90, liver L-02, and stomach GES-1 cells. Cells were seeded in 96-well plates and treated with different concentrations of shikonin, 5-FU, HEDMNQ, and HHDMNQ (0, 1, 3, 10, 30, and 100 μM) for 24 h, and then the cell viabilities were determined by the CCK-8 assay. (c) Cell viabilities of A549, NCI-H23, and NCI-H460 cells. (d) Cell viabilities of normal lung IMR-90, liver L-02, and stomach GES-1 cells. Cells were seeded in 96-well plates and treated with shikonin, 5-FU, HEDMNQ, and HHDMNQ for 3, 6, 12, 24, and 36 h, and then the cell viabilities were determined by the CCK-8 assay. Data were collected from three individual experiments (, , ).

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