Suppression of Inflammatory and Fibrotic Signals by Cinnamon (Cinnamomum cassia) and Cinnamaldehyde in Cyclophosphamide-Induced Overactive Bladder in Mice

Figure 5

Effects of CNP and CNA on changes in the signaling of tissue fibrosis including SCF/c-Kit and β-catenin/α-SMA in cyclophosphamide- (CYP-, 300 mg/kg) induced OAB mice 24 hours after CYP induction. Typical confocal images from bladder slices of animal groups described in Figure 3 show changes in colocalization of (a) SCF (green, G) with c-Kit (red, R), (b) β-catenin (red, R) with α-SMA (green, G) and DAPI (blue, B) (a marker for nuclei). Arrows indicate colocalization of SCF/c-Kit and β-catenin/α-SMA. Statistical results of colocalization stain positive area (%) of the whole image. Data are presented as mean ± SEM (n = 5). ^†, compared with control or CYP + Veh group, respectively, by one-way ANOVA followed by S-N-K post hoc t-test for multiple comparisons.

(a)

(b)