Effects of Water Extract of Cynanchum paniculatum (Bge.) Kitag. on Different Breast Cancer Cell Lines

Table 4

Differential sensitivity to migration is correlated to genomic characteristics.

Breast cancer cell lines	ER	PR	HER	Direct CP inhibit migration	Direct CP reduced MMP-2 expression	Sup-CP inhibit migration	Sup-CP reduced MMP-2
MDA-MB-231	−	−	−	Y	Y	Y	Y
SK-Br-3	−	−	+	N	N	N	N
MCF-7	+	+	−	Y	N	Y	Y
ZR-75-1	+	+	+	N	N	N	N

The different genomic characteristics were listed in an array and compared to two traits (shown in the right four columns). The first result column demonstrated that when the cells were treated with similar CP extract, MDA-MB-231 and MCF−7 (both HER(−)), the migration of cells were inhibited, hence the Y (yes) for this phenotype, while SK-Br−3 and ZR−75−1 (both HER(+)) were not inhibited, hence the N (no). The second result column summarizes the results demonstrated that when the cells were treated with similar CP extract, only MDA−MB−231 cells demonstrated reduced MMP-2 level. There is no association to the genotypes. The third column demonstrated that when the cells were treated with similar CP extract, MDA−MB−231 and MCF-7 (both HER(−)), the migration of cells were inhibited by treatment of CP-treated conditioned medium, while SK−Br−3 and ZR−75−1 (both HER(+)) were not. The fourth column demonstrated that when the cells were treated with CP-treated conditioned medium, MDA−MB−231 and MCF-7 (both HER(−)), the MMP-2 levels were inhibited, while SK−Br−3 and ZR−75−1 (both HER(+)) were not. For details of the results, please see Figures 5–7 and respective text.