[Retracted] The Number of Intraoperative Intestinal Venous Circulating Tumor Cells Is a Prognostic Factor for Colorectal Cancer Patients

Figure 3

Immunostaining profiles of CTCs isolated from the IVB of CRC patients. CTCs from these patients underwent imFISH staining, with CTCs being identified as cells staining positive for anti-human CEP8, but negative for antihuman CD45. (a) Positive control group. (b) CTC = 0. (c) CTC = 22. (d) CTC = 12. (e) CTC = 6. (f) CTC = 1. Identified CTCs exhibited normal morphological findings upon microscopic evaluation (400x). As per the above Figures 3(a)–3(f), space is limited, not showing all the results. (g) The mean viability of enriched tumor cells (yellow) and WBCs (red) was 98.67 ± 0.88% and 95.20 ± 1.38% (mean ± SD), respectively. Data are representative images of three independent experiments. Asterisks were marked to show the significance of tumor cells group compared with WBCs group (P < 0.001). CTCs, circulating tumor cells; imFISH, immunofluorescence in situ hybridization; CD45, leukocyte-specific antibodies; CEP8, human chromosome 8 specific sequence; DAPI, 4′,6-diamidino-2-phenylindole; MERGE, CD45 + CEP8 + DAPI; WBCs, whole blood cells.