Enzyme Research

Enzyme Research / 2015 / Article / Tab 6

Research Article

Immobilization of Papain on Chitin and Chitosan and Recycling of Soluble Enzyme for Deflocculation of Saccharomyces cerevisiae from Bioethanol Distilleries

Table 6

Total activity of papain in several steps, according to each protocol of immobilization.

Immobilization protocolaTotal enzyme in solutionEnzyme solution after the immobilization process1st pellets washing-water (100 mL)2nd pellets washing-water (1000 mL)Active immob. enzyme (U)c

A355.14215.88 (14)b74.00.01.53 (6.5)d
B481.34333.48 (14)136.00.00.72 (6.5)
C416.86122.46 (13)148.066.70.43 (6.5)
D316.00132.66 (18)48.00.00.00 (7.5)
E360.94240.79 (18)70.331.00.00 (7.5)
F424.46225.54 (18)87.00.00.10 (7.5)
A-5 h206.5055.97 (39)0.00.05.76 (2.78)
B-5 h180.65155.61 (26)0.00.03.94 (3.17)
C-5 h201.00138.13 (33)0.00.02.24 (2.70)
A-12 h184.7534.59 (30)0.00.02.21 (2.66)
B-12 h197.506.65 (25)0.00.03.72 (2.86)
C-12 h197.7529.92 (34)0.00.02.34 (2.84)

aProtocols in Tables 1 and 2; bremaining volume (mL) obtained from each immobilization is indicated in parentheses; ctotal units of immobilized enzyme, and dwet weight (g) of pellets.

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