Enzyme Research

Enzyme Research / 2015 / Article / Tab 7

Research Article

Immobilization of Papain on Chitin and Chitosan and Recycling of Soluble Enzyme for Deflocculation of Saccharomyces cerevisiae from Bioethanol Distilleries

Table 7

Determination of yield of active immobilized papain and other parameters.

Immobilization protocolsaEnzyme activity after immobilization (in solution) (%)Enzyme activity involved in immobilization (%)Yieldc of active immobilized enzyme (%)Yieldd of immobilized protein (%) and immob. protein on support (mg⋅g−1)Enzymatic activity in support (U/g)

A81.6218.372.340.24 (0.12)0.23
B97.532.466.070.3 (0.148)0.11
C64.8835.110.290.3 (0.15)0.06
D57.1742.820.0013.56 (6.73)0.00
E86.1813.810.0043.58 (21.63)0.00
F73.6326.360.0867.41 (33.46)0.013
A-5 h27.1072.893.8235.00 (24.60)2.07
B-5 h86.1313.8615.739.06 (4.50)1.24
C-5 h68.7231.273.5645.9 (23.90)0.82
A-12 h18.7281.271.4744.06 (30.80)0.83
B-12 h3.3696.631.9445.9 (23.90)1.30
C-12 h15.1384.861.3946.64 (29.40)0.82

aProtocols in Tables 1 and 2; bcalculated as B × A−1  × 100 where B is the total activity of the enzyme remaining in solution after immobilization process; A is the total activity of the enzyme added to the support; ccalculated as total activity of immobilized enzyme (U) × (AB)−1 × 100; dcalculated as C × D−1  × 100 where C is the immobilized protein (g); D is total protein added on support (g), total protein remaining in solution after the immobilization process (g).

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