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Gastroenterology Research and Practice
Volume 2017 (2017), Article ID 1526981, 8 pages
https://doi.org/10.1155/2017/1526981
Research Article

A Downmodulated MicroRNA Profiling in Patients with Gastric Cancer

Department of Gastroenterology, Ruikang Hospital, Guangxi Traditional Chinese Medical University, Nanning, Guangxi 530011, China

Correspondence should be addressed to Yuanneng Chen; moc.nuyila@86606nyc

Received 10 December 2016; Revised 24 February 2017; Accepted 9 March 2017; Published 4 May 2017

Academic Editor: Nicola Silvestris

Copyright © 2017 Tao Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. Here, we aim to investigate the microRNA (miR) profiling in human gastric cancer (GC). Methods. Tumoral and matched peritumoral gastric specimens were collected from 12 GC patients who underwent routine surgery. A high-throughput miR sequencing method was applied to detect the aberrantly expressed miRs in a subset of 6 paired samples. The stem-loop quantitative real-time polymerase chain reaction (qRT-PCR) assay was subsequently performed to confirm the sequencing results in the remaining 6 paired samples. The profiling results were also validated in vitro in three human GC cell lines (BGC-823, MGC-803, and GTL-16) and a normal gastric epithelial cell line (GES-1). Results. The miR sequencing approach detected 5 differentially expressed miRs, hsa-miR-132-3p, hsa-miR-155-5p, hsa-miR-19b-3p, hsa-miR-204-5p, and hsa-miR-30a-3p, which were significantly downmodulated between the tumoral and peritumoral GC tissues. Most of the results were further confirmed by qRT-PCR, while no change was observed for hsa-miR-30a-3p. The in vitro finding also agreed with the results of both miR sequencing and qRT-PCR for hsa-miR-204-5p, hsa-miR-155-5p, and hsa-miR-132-3p. Conclusion. Together, our findings may serve to identify new molecular alterations as well as to enrich the miR profiling in human GC.