Identification and Characterization of Wheat Streak Mosaic Virus Isolates in Wheat-Growing Areas in Brazil

Table 1

Samples of wheat (Triticum aestivum L.) showing mosaic symptoms collected in ten counties from the state of Rio Grande do Sul.

Sample	Latitude	Longitude	Geographic origin	Year	CP gene amplification*
329	−28 24′57,20000′′	−55 00′29,60000′′	São Luiz Gonzaga	2009	—
358	−27 29′49,60000′′	−52 54′07,40000′′	Nonoai	2009	—
363	−27 17′′10,20000′′	−52 43′25,60000′′	Nonoai	2009	—
451	−28 24′57,20000′′	−55 00′29,60000′′	São Luiz Gonzaga	2009	—
485	−28 13′49,89000′′	−52 24′15,99000′′	Passo Fundo (Greenhouse)	2010	—
486	−28 13′49,89000′′	−52 24′15,99000′′	Passo Fundo (Greenhouse)	2010	948 bp
763	−27 19′36,23677′′	−52 46′18,55250′′	Nonoai	2010	—
765	−27 19′36,23677′′	−52 46′18,55250′′	Nonoai	2010	—
817	−28 06′35,02305′′	−52 54′29,57809′′	Alm. Tamandaré do Sul	2010	—
823	−27 45′57,35304′′	−53 27′29,49793′′	Palmeira das Missões	2010	—
837	−28 23′59,00000′′	−54 39′52,00000′′	São Miguel das Missões	2010	—
839	−28 24′57,22000′′	−55 00′29,41628′′	São Luiz Gonzaga	2010	—
852	−28 23′00,40000′′	−54 02′56,00000′′	Ijuí	2010	—
862	−27 45′57,35304′′	−53 27′29,49793′′	Palmeira das Missões	2010	—
879	−28 24′57,22908′′	−55 00′29,41628′′	São Luiz Gonzaga	2010	—
910	−28 13′31,15000′′	−52 24′19,31000′′	Passo Fundo	2010	—
911	−28 14′10,94000′′	−52 24′26,67000′′	Passo Fundo	2010	—
912	−28 13′49,89000′′	−52 24′15,99000′′	Passo Fundo (Greenhouse)	2010	948 bp
913	−28 13′33,99000′′	−52 23′23,75000′′	Passo Fundo	2010	—
914	−28 13′33,99000′′	−52 23′23,75000′′	Passo Fundo	2010	—
915	−28 13′49,89000′′	−52 24′15,99000′′	Passo Fundo (Greenhouse)	2011	948 bp
1211	−28 06′35,02305′′	−52 54′29,57809′′	Alm. Tamandaré do Sul	2011	—
1212	−28 06′59,00000′′	−52 57′02,00000′′	Alm. Tamandaré do Sul	2011	—
1213	−28 01′08,80966′′	−53 05′52,31161′′	Chapada	2011	—
1214	−27 58′44,00000′′	−53 08′53,00000′′	Chapada	2011	—
1215	−27 57′16,00000′′	−53 14′58,00000′′	Palmeira das Missões	2011	—
1216	−27 51′59,00000′′	−53 19′31,00000′′	Palmeira das Missões	2011	—
1217	−27 45′57,35304′′	−53 27′29,49793′′	Palmeira das Missões	2011	—
1220	−27 52′52,94114′′	−54 26′02,50637′′	Santa Rosa	2011	—
1226	−28 29′35,59530′′	−54 33′37,15222′′	São Miguel das Missões	2011	—
1227	−28 23′59,00000′′	−54 39′52,00000′′	São Miguel das Missões	2011	—
1228	−28 24′29,00000′′	−54 42′15,0000′′	São Miguel das Missões	2011	—
1229	−28 25′02,00000′′	−54 49′21,0000′′	São Luiz Gonzaga	2011	—
1230	−28 25′40,00000′′	−54 57′57,00000′′	São Luiz Gonzaga	2011	—
1231	−28 30′05,00000′′	−54 57′08,00000′′	São Luiz Gonzaga	2011	—
1233	−28 24′57,22908′′	−55 00′29,41628′′	São Luiz Gonzaga	2011	948 bp
1234	−28 29′03,0000′′	−55 13′12,00000′′	Sto. Antônio das Missões	2011	—
1251	−28 13′31,15000′′	−52 24′19,31000′′	Passo Fundo	2011	—
1254	−28 13′33,99000′′	−52 23′23,75000′′	Passo Fundo	2011	948 bp
1256	−28 13′49,89000′′	−52 24′15,99000′′	Passo Fundo (Greenhouse)	2011	948 bp

∗Amplification of a “948 pb” or “—” PCR negative samples.