Gas Chromatographic/Mass Spectrometric Analysis of Volatile Metabolites in Bovine Vaginal Fluid and Assessment of Their Bioactivity

Table 2

Bioactivity of oestrus-specific compounds.

S. no.	Oestrus-specific compounds	Duration of flehmen (seconds)	Number of mounts
1	Acetic acid	5.52 ± 0.02	8.40 ± 0.46
2	Propionic acid	5.58 ± 0.22	8.80 ± 0.52
3	Trimethylamine	4.20 ± 0.08	5.85 ± 0.52
4	Phenol	2.63 ± 0.02	3.96 ± 0.40
5	Mixture of acetic acid and propionic acid	6.96 ± 0.52	8.84 ± 0.52
6	Mixture of acetic acid and trimethylamine	4.68 ± 0.54	6.08 ± 0.40
7	Mixture of propionic acid and trimethylamine	5.13 ± 0.33	5.60 ± 0.06
8	Mixture of acetic acid, propionic acid and trimethylamine	8.60 ± 0.01	10.94 ± 0.87
9	Phosphonic acid (negative control; non-oestrus sample)	2.19 ± 0.33	3.63 ± 0.36

Values are mean ± S.E of six observations.