Gas Chromatographic/Mass Spectrometric Analysis of Volatile Metabolites in Bovine Vaginal Fluid and Assessment of Their Bioactivity
Table 3
Analysis of variance (ANOVA) with post hoc comparison (one-way), Homogeneous subsets (duration of flehmen), Homogeneous subsets (mounting behaviour).
(a)
ANOVA
Sum of squares
df
Mean squares
VAR 00001 Duration of flehmen
Between squares
189.995
8
23.749
76.874
0.000**
Within groups
13.902
45
.309
Total
203.897
53
VAR 00003 Mounting behaviour
Between squares
294.012
8
36.752
134.779
0.000**
Within groups
12.271
45
.273
Total
306.283
53
**Level of significance at ().
(b)
VAR 00001 DUNCAN
Subset for alpha
VAR 00002
1
2
3
4
5
6
Phosphonic acid
6
2.1917
Phenol
6
2.6333
Trimethylamine
6
4.2083
Acetic + TMA
6
4.6817
4.6817
Propionic + TMA
6
5.1367
5.1367
Acetic acid
6
5.5250
Propionic acid
6
5.5800
Acetic + propionic
6
6.9600
Acetic + propionic + TMA
6
8.6067
Significant
0.176
0.147
0.163
0.199
1.000
1.000*
Means for groups in homogenous subsets are displayed comparison of means using DMRT. The DMRT test showed that the mixture of acetic acid, propionic acid, and trimethylamine was found to be significant () compared to those of acetic acid, propionic acid, Trimethylamine, phosphonic acid, and phenol separately as well as combinations.
(c)
VAR 00001 DUNCAN
Subset for alpha
VAR 00002
1
2
3
4
Phosphonic acid
6
3.6350
Phenol
6
3.9683
Propionic + TMA
6
5.6067
Trimethylamine
6
5.8567
Acetic + TMA
6
6.0817
Acetic acid
6
8.4033
Acetic + propionic
6
8.8467
Propionic acid
6
8.8867
Acetic + propionic + TMA
6
10.9400
Significant
0.275
0.144
0.137
1.000*
Means for groups in homogenous subsets are displayed comparison of means using DMRT. The DMRT test showed that the mixture of acetic acid, propionic acid, and trimethylamine was found to be significant () compared to those of acetic acid, propionic acid, trimethylamine, phosphonic acid, and phenol separately as well as combinations. Abbreviation: TMA: trimethylamine.