Research Article

Gas Chromatographic/Mass Spectrometric Analysis of Volatile Metabolites in Bovine Vaginal Fluid and Assessment of Their Bioactivity

Table 3

Analysis of variance (ANOVA) with post hoc comparison (one-way), Homogeneous subsets (duration of flehmen), Homogeneous subsets (mounting behaviour).
(a)

ANOVA
Sum of squaresdfMean squares

VAR 00001 Duration of flehmen Between squares189.995823.74976.8740.000**
Within groups13.90245.309
Total203.89753

VAR 00003 Mounting behaviour Between squares294.012836.752134.7790.000**
Within groups12.27145.273
Total306.28353

**Level of significance at ( ).
(b)

VAR 00001
DUNCAN

Subset for alpha
VAR 00002 123456

Phosphonic acid62.1917
Phenol6 2.6333
Trimethylamine64.2083
Acetic + TMA64.68174.6817
Propionic + TMA65.13675.1367
Acetic acid65.5250
Propionic acid65.5800
Acetic + propionic66.9600
Acetic + propionic + TMA68.6067
Significant 0.176 0.147 0.1630.1991.0001.000*

Means for groups in homogenous subsets are displayed comparison of means using DMRT. The DMRT test showed that the mixture of acetic acid, propionic acid, and trimethylamine was found to be significant ( ) compared to those of acetic acid, propionic acid, Trimethylamine, phosphonic acid, and phenol separately as well as combinations.
(c)

VAR 00001 DUNCAN

Subset for alpha
VAR 00002 1234

Phosphonic acid 63.6350
Phenol63.9683
Propionic + TMA65.6067
Trimethylamine65.8567
Acetic + TMA66.0817
Acetic acid68.4033
Acetic + propionic68.8467
Propionic acid68.8867
Acetic + propionic + TMA 610.9400
Significant0.2750.1440.1371.000*

Means for groups in homogenous subsets are displayed comparison of means using DMRT. The DMRT test showed that the mixture of acetic acid, propionic acid, and trimethylamine was found to be significant ( ) compared to those of acetic acid, propionic acid, trimethylamine, phosphonic acid, and phenol separately as well as combinations. Abbreviation: TMA: trimethylamine.