Research Article

Quantification of Antioxidant Phenolic Compounds in a New Chrysanthemum Cultivar by High-Performance Liquid Chromatography with Diode Array Detection and Electrospray Ionization Mass Spectrometry

Table 3

Linear range, regression equation, correlation coefficients, LODs, and LOQs of the compounds.

CompoundRegression equationCorrelation coefficient (r2)LOD (μg/mL)LOQ (μg/mL)

Acacetin (1)y = 11.349x + 2.5200.99960.0570.173
Apigenin (2)y = 36.953x − 46.9800.99920.5751.742
Luteolin (3)y = 23.094x + 5.2200.99950.2440.738
Acacetin-7-O-β-glucoside (4)y = 14.277x + 20.1200.99910.2060.624
Apigenin-7-O-β-glucoside (5)y = 33.317x − 9.1700.99920.5421.644
Luteolin-7-O-β-glucoside (6)y =14.105x − 16.1800.99930.2670.811
Linarin (7)y = 21.849x − 21.3400.99940.7162.168
Chlorogenic acid (8)y = 18.675x − 17.9070.99940.1780.539
Mixture of 1,4-, 1,5-, and 3,5-DCQAs (911)y = 22.766x − 25.320.99910.6011.822

y = peak area, x = concentration (μg/ml), = slope, = intercept; limit of detection (LOD): 3.3 × (SD of the response/slope of the calibration curve); limit of quantification (LOQ): 10 × (SD of the response/slope of the calibration curve).