Quantification of Antioxidant Phenolic Compounds in a New Chrysanthemum Cultivar by High-Performance Liquid Chromatography with Diode Array Detection and Electrospray Ionization Mass Spectrometry
Table 3
Linear range, regression equation, correlation coefficients, LODs, and LOQs of the compounds.
Compound
Regression equation
Correlation coefficient (r2)
LOD (μg/mL)
LOQ (μg/mL)
Acacetin (1)
y = 11.349x + 2.520
0.9996
0.057
0.173
Apigenin (2)
y = 36.953x − 46.980
0.9992
0.575
1.742
Luteolin (3)
y = 23.094x + 5.220
0.9995
0.244
0.738
Acacetin-7-O-β-glucoside (4)
y = 14.277x + 20.120
0.9991
0.206
0.624
Apigenin-7-O-β-glucoside (5)
y = 33.317x − 9.170
0.9992
0.542
1.644
Luteolin-7-O-β-glucoside (6)
y =14.105x − 16.180
0.9993
0.267
0.811
Linarin (7)
y = 21.849x − 21.340
0.9994
0.716
2.168
Chlorogenic acid (8)
y = 18.675x − 17.907
0.9994
0.178
0.539
Mixture of 1,4-, 1,5-, and 3,5-DCQAs (9–11)
y = 22.766x − 25.32
0.9991
0.601
1.822
y = peak area, x = concentration (μg/ml), = slope, = intercept; limit of detection (LOD): 3.3 × (SD of the response/slope of the calibration curve); limit of quantification (LOQ): 10 × (SD of the response/slope of the calibration curve).