Simple Quantification of Pentosidine in Human Urine and Plasma by High-Performance Liquid Chromatography

Table 1

Mean response factors, coefficients of variation (CV), accuracy, and recovery of pentosidine at various concentrations in human urine.

Concentration (nM)	Response factor ± SD (% CV)	(%)	(%)
1	81986 ± 7746 (9.45)	99.6	112
2	73662 ± 884 (1.20)	89.5	97.2
3	80179 ± 3599 (4.49)	97.4	109
4	83676 ± 5233 (6.25)	102	111
5	87057 ± 1527 (1.75)	106	114
10	83147 ± 1457 (1.75)	101	112
20	84339 ± 2234 (2.65)	102	112
50	84359 ± 1510 (1.79)	103	105

Measurements were conducted three times in separate days; the response factor was calculated by subtracting nonspiked response factor from the spiked standard response factor followed by dividing by the spiked concentration; data are expressed as mean response factor ± standard deviation (SD) ; values in bracket are intraday coefficients of variation (CV). ; recovery compared with water.