Simple Quantification of Pentosidine in Human Urine and Plasma by High-Performance Liquid Chromatography

Table 2

Mean response factors, coefficients of variation (CV), accuracy, and recovery of pentosidine at various concentrations in human plasma.

Concentration (nM)	Response factor ± SD (% CV)	(%)	(%)
1	31503 ± 719 (2.28)	87.9	41.6
2	36813 ± 104 (0.28)	103	54.0
5	36456 ± 1154 (3.16)	102	51.4
10	36417 ± 673 (1.85)	102	55.7
20	35682 ± 1242 (3.48)	99.6	57.3
30	36374 ± 682 (1.88)	102	53.0
40	36677 ± 461 (1.26)	102	53.6
50	36638 ± 1737 (4.74)	102	50.5

Measurements were conducted three times in separate days; The response factor was calculated by subtracting nonspiked response factor from the spiked standard response factor followed by dividing by the spiked concentration; data are expressed as mean response factor ± standard deviation (SD) (); values in bracket are intraday coefficients of variation (CV). ; recovery compared with water.